Structure of PDB 4tmu Chain A Binding Site BS01

Receptor Information

>4tmu Chain A (length=511) Species: 290339 (Cronobacter sakazakii ATCC BAA-894)

AEVYSQETLAKQVLQETFGYQQFRPGQATIIDAVLEGRDCLVVMPTGGGK
SLCYQIPALVKTGLTIVVSPLISLMKDQVDQLLANGVAAACLNSTQSREE
QQAVLAGCRTGQVRLLYIAPERLMMDNFIDTLGYWDLAMVAVDEAHCISQ
WGHDFRPEYAALGQLRARFPAVPFMALTATADDTTRRDIVRLLGLDDPLI
EISSFDRPNIRYMLMEKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTA
ARLQSRGISAAAYHAGLEHEVRASVQEKFQRDDLQIVVATVAFGMGINKP
NVRFVVHFDIPRNIESYYQETGRAGRDGLPAEAMLFYDPADMAWLRRCLE
EKAPGPLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEGRQAPCGNCDIC
LDPPRRYDGLVDAQKALSAIARVEQRFGMGYVVEVLRGANNQRIRELGHD
KLKVYGIGRDQSQEHWVSVIRQLIHLGVVTQNIAQHSALQLTEAARPFLR
GEAPLMLAVPR

Ligand information

>4tmu Chain B (length=29)

gtagaattgaaattctaccggtgccttac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4tmu Structural mechanisms of DNA binding and unwinding in bacterial RecQ helicases.
• Resolution: 2.4 Å
• Binding residue (original residue number in PDB): L74 I75 N96 S97 E124 R125 W154 F158 R159 P223 L224 S245 R246 R259 H267 A268 T293 V294 R315 W347 M432 G433 A442 N443 N444 R446 H489 S490
• Binding residue (residue number reindexed from 1): L71 I72 N93 S94 E121 R122 W151 F155 R156 P220 L221 S242 R243 R256 H264 A265 T290 V291 R312 W344 M429 G430 A439 N440 N441 R443 H486 S487
• Binding affinity: PDBbind-CN: Kd=1.5nM

Enzymatic activity

• Enzyme Commision number: 5.6.2.4: DNA 3'-5' helicase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003678 DNA helicase activity
• GO:0004386 helicase activity
• GO:0005524 ATP binding
• GO:0043138 3'-5' DNA helicase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0009432 SOS response

External links

• PDB: RCSB:4tmu, PDBe:4tmu, PDBj:4tmu
• PDBsum: 4tmu
• PubMed: 25831501
• UniProt: A7MQK9