Structure of PDB 4pz6 Chain A Binding Site BS01

Receptor Information

>4pz6 Chain A (length=373) Species: 284812 (Schizosaccharomyces pombe 972h-)

SMAPSEKDIEEVSVPGVLAPRDDVRVLKTRIAKLLGTSPDTFPGSQPVSF
SKKHLQALKEKNYFVCEKSDGIRCLLYMTEHPRYENRPSVYLFDRKMNFY
HVEKIFYPVENDKSGKKYHVDTLLDGELVLDIYPGGKKQLRYLVFDCLAC
DGIVYMSRLLDKRLGIFAKSIQKPLDEYTKTHMRETAIFPFLTSLKKMEL
GHGILKLFNEVIPRLRHGNDGLIFTCTETPYVSGTDQSLLKWKPKEMNTI
DFMLKLEFAQPEEGDIDYSAMPEFQLGVWEGRNMYSFFAFMYVDEKEWEK
LKSFNVPLSERIVECYLDDENRWRFLRFRDDKRDANHISTVKSVLQSIED
GVSKEDLLKEMPIIREAYYNRKK

Ligand information

>4pz6 Chain Q (length=8) Species: 284812 (Schizosaccharomyces pombe 972h-)

SPSYSPTS

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4pz6 How an mRNA capping enzyme reads distinct RNA polymerase II and Spt5 CTD phosphorylation codes.
• Resolution: 2.406 Å
• Binding residue (original residue number in PDB): R157 D160 G164 I165 L194 R364 Y368
• Binding residue (residue number reindexed from 1): R158 D161 G165 I166 L195 R365 Y369

Enzymatic activity

• Enzyme Commision number: 2.7.7.50: mRNA guanylyltransferase.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004484 mRNA guanylyltransferase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0005525 GTP binding
• GO:0016779 nucleotidyltransferase activity
• GO:0099122 RNA polymerase II C-terminal domain binding

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006370 7-methylguanosine mRNA capping

Cellular Component

• GO:0005634 nucleus
• GO:0031533 mRNA capping enzyme complex

External links

• PDB: RCSB:4pz6, PDBe:4pz6, PDBj:4pz6
• PDBsum: 4pz6
• PubMed: 24939935
• UniProt: P40997|MCE1_SCHPO mRNA-capping enzyme subunit alpha (Gene Name=ceg1)