Structure of PDB 4n5s Chain A Binding Site BS01

Receptor Information

>4n5s Chain A (length=542) Species: 271 (Thermus aquaticus)

SPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPY
KALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEG
VARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSA
VLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQ
LERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELT
KLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSSSDPNLQNIPVRTP
LGQRIRRAFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIH
TETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEA
QAFIERYFQSFPKVRAWLEKTLEEGRRRGYVETLFGRRRYVPDLEARVKS
VREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVL
EAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAK

Ligand information

>4n5s Chain B (length=12)

gaccacggcgcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4n5s A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KlenTaq1 DNA Polymerase.
• Resolution: 1.67 Å
• Binding residue (original residue number in PDB): R487 T506 K508 T509 S513 T514 S515 A516 R536 K540 Y545 R573 Q582 N583 I584 P585 V586 R587 R660 V783 H784
• Binding residue (residue number reindexed from 1): R198 T217 K219 T220 S224 T225 S226 A227 R247 K251 Y256 R284 Q293 N294 I295 P296 V297 R298 R371 V494 H495

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0001882 nucleoside binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair

External links

• PDB: RCSB:4n5s, PDBe:4n5s, PDBj:4n5s
• PDBsum: 4n5s
• PubMed: 25537790
• UniProt: P19821|DPO1_THEAQ DNA polymerase I, thermostable (Gene Name=polA)