Structure of PDB 4lgt Chain A Binding Site BS01

Receptor Information

>4lgt Chain A (length=251) Species: 83333 (Escherichia coli K-12)

MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEVTPGLKI
RIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRG
ARWIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDD
AKLRDLSRGVQLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLW
EAVGVQVSRLIRVRYGDIPLPKGLPRGGWTELDLAQTNYLRELVELPPET
S

Ligand information

>4lgt Chain E (length=21)

agaacgucgugagacaguucg

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Receptor-Ligand Complex Structure

Structure summary

• PDB: 4lgt The mechanism of pseudouridine synthases from a covalent complex with RNA, and alternate specificity for U2605 versus U2604 between close homologs.
• Resolution: 1.3 Å
• Binding residue (original residue number in PDB): K4 Q6 K7 S16 R17 R18 E21 L38 G39 D40 R41 L79 T81 R82 R88 R102 G107 R108 L109 D110 V111 G123 N127 H131 P132 S133 E135 R138 Y140 G191 N193 R194 V196 L210 R212 Y215
• Binding residue (residue number reindexed from 1): K4 Q6 K7 S16 R17 R18 E21 L38 G39 D40 R41 L79 T81 R82 R88 R102 G107 R108 L109 D110 V111 G123 N127 H131 P132 S133 E135 R138 Y140 G191 N193 R194 V196 L210 R212 Y215

Enzymatic activity

• Enzyme Commision number: 5.4.99.22: 23S rRNA pseudouridine(2605) synthase.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0009982 pseudouridine synthase activity
• GO:0016866 intramolecular transferase activity

Biological Process

• GO:0001522 pseudouridine synthesis
• GO:0009451 RNA modification

External links

• PDB: RCSB:4lgt, PDBe:4lgt, PDBj:4lgt
• PDBsum: 4lgt
• PubMed: 24214967
• UniProt: P37765|RLUB_ECOLI Ribosomal large subunit pseudouridine synthase B (Gene Name=rluB)