Structure of PDB 4jrp Chain A Binding Site BS01

Receptor Information
>4jrp Chain A (length=460) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
QQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADD
YLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVR
FDDEVTRNIFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPE
NDDGLPSFRLEHLTKANGIEHDAMADVYATIAMAKLVKTRQPRLFDYLFT
HRNKHKLMALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVI
MVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLA
QANTLRPEDADRLGINRQHCLDNLKILRENPQVREKVVAIFAEASDNVDA
QLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKRIEKLLFNYRARNF
PGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLVQQYADDKEKVALLK
ALWQYAEEIV
Ligand information
Receptor-Ligand Complex Structure
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PDB4jrp Crystal structures of Escherichia coli exonuclease I in complex with single-stranded DNA provide insights into the mechanism of processive digestion.
Resolution1.95 Å
Binding residue
(original residue number in PDB)
E17 T18 G20 T21 A63 I66 T67 Y102 N103 F107 R113 Y124 W128 S133 R142 F164 R165 L166 H181 R212 K214 G234 M235 G237 A238 N255 N257 L283 Y284 T285 A286 K287 N304 S360 Y368 N369 F371
Binding residue
(residue number reindexed from 1)
E11 T12 G14 T15 A57 I60 T61 Y96 N97 F101 R107 Y118 W122 S127 R136 F158 R159 L160 H171 R202 K204 G224 M225 G227 A228 N245 N247 L273 Y274 T275 A276 K277 N294 S345 Y353 N354 F356
Enzymatic activity
Enzyme Commision number 3.1.11.1: exodeoxyribonuclease I.
Gene Ontology
Molecular Function
GO:0000175 3'-5'-RNA exonuclease activity
GO:0000287 magnesium ion binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003697 single-stranded DNA binding
GO:0004527 exonuclease activity
GO:0004529 DNA exonuclease activity
GO:0005515 protein binding
GO:0008310 single-stranded DNA 3'-5' DNA exonuclease activity
GO:0046872 metal ion binding
GO:0051575 5'-deoxyribose-5-phosphate lyase activity
Biological Process
GO:0006259 DNA metabolic process
GO:0006274 DNA replication termination
GO:0006281 DNA repair
GO:0006308 DNA catabolic process

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Molecular Function

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Biological Process
External links
PDB RCSB:4jrp, PDBe:4jrp, PDBj:4jrp
PDBsum4jrp
PubMed23609540
UniProtP04995|EX1_ECOLI Exodeoxyribonuclease I (Gene Name=sbcB)

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