Structure of PDB 4ecs Chain A Binding Site BS01

Receptor Information

>4ecs Chain A (length=430) Species: 9606 (Homo sapiens)

GPHMATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGI
IAVSYEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASV
EVMEIMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYI
EGLPQGPTVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRA
AIERETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPI
RKIRSLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMC
RGIEHDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERL
TKDRNDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTV
IKNCNTSGIQTEWSPPLTMLFLCATKFSAS

Ligand information

>4ecs Chain T (length=12)

cattatgacgct

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4ecs Watching DNA polymerase eta make a phosphodiester bond
• Resolution: 1.951 Å
• Binding residue (original residue number in PDB): Q38 Y39 W42 S62 K86 L89 R93 K293 P316 K317 T318 G320 C321 S322 K323 N324 P326 G327 R351
• Binding residue (residue number reindexed from 1): Q41 Y42 W45 S65 K89 L92 R96 K291 P314 K315 T316 G318 C319 S320 K321 N322 P324 G325 R349

Enzymatic activity

• Catalytic site (original residue number in PDB): D13 M14 Y52 R55 R61 S113 D115 E116 K231
• Catalytic site (residue number reindexed from 1): D16 M17 Y55 R58 R64 S116 D118 E119 K229
• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:4ecs, PDBe:4ecs, PDBj:4ecs
• PDBsum: 4ecs
• PubMed: 22785315
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)