Structure of PDB 4cvk Chain A Binding Site BS01

Receptor Information

>4cvk Chain A (length=438) Species: 208964 (Pseudomonas aeruginosa PAO1)

MLEPLRLSQLTVALDARLIGEDAVFSAVSTDSRAIGPGELFIALSGPRFD
GHDYLAEVAAKGAVAALVEREVAAPLPQLLVRDTRAALGRLGALNRRKFT
GPLAAMTGSSGKTTVKEMLASILRTQAGDAESVLATRGNLNNDLGVPLTL
LQLAPQHRSAVIELGASRIGEIAYTVELTRPHVAIITNAFGGPEKIVEAK
GEILEGLAADGTAVLNLDDKAFDTWKARASGRPLLTFSLDRPQADFRAAD
LQRDARGCMGFRLQGVAGEAQVQLNLLGRHNVANALAAAAAAHALGVPLD
GIVAGLQALQPVKGRAVAQLTASGLRVIDDSYNANPASMLAAIDILSGFS
GRTVLVLGDMGAEQAHREVGAYAAGKVSALYAVGPLMAHAVQAFGATGRH
FADQASLIGALATEDPTTTILIKGSRSAAMDKVVAALC

Ligand information

>4cvk Chain B (length=3) Species: 32630 (synthetic construct)

AEt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4cvk Pamurf in Complex with Udp-Murnac-Tripeptide (Mdap)
• Resolution: 1.92 Å
• Binding residue (original residue number in PDB): K113 N140 L141 N142 N143 G146 A167 S168 E172
• Binding residue (residue number reindexed from 1): K112 N139 L140 N141 N142 G145 A166 S167 E171

Enzymatic activity

• Catalytic site (original residue number in PDB): K113 T114 D144 E164
• Catalytic site (residue number reindexed from 1): K112 T113 D143 E163
• Enzyme Commision number: 6.3.2.10: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase.

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008766 UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
• GO:0016874 ligase activity
• GO:0016881 acid-amino acid ligase activity
• GO:0046872 metal ion binding
• GO:0047480 UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase activity

Biological Process

• GO:0008360 regulation of cell shape
• GO:0009058 biosynthetic process
• GO:0009252 peptidoglycan biosynthetic process
• GO:0051301 cell division
• GO:0071555 cell wall organization

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4cvk, PDBe:4cvk, PDBj:4cvk
• PDBsum: 4cvk
• UniProt: Q9HVZ7