Structure of PDB 4ari Chain A Binding Site BS01

Receptor Information
>4ari Chain A (length=821) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
HMQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLH
MGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAP
WTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLV
YKKTSAVEIPQWFIKITAYADELLNDLDKLDHWPDTVKTMQRNWIGRSEG
VEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELA
AFIDECRNTEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVP
GHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNG
LDHEAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLED
GTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDT
FDTFMESSWYYARYTCPQYKEGMLDSEAANYWLPVDIYIGGIEHAIMHLL
YFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPV
DAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSKNNGIDPQVMVERYGA
DTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAAL
NVDALTENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKA
PTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPV
ADEKAMVEDSTLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYL
DGVTVRKVIYVPGKLLNLVVG
Ligand information
>4ari Chain B (length=80) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gcccggaugguggaaucgguagacacaagggaucccucggcguucgcgcu
gugcggguucaagucccgcuccggguacca
<<<<<<<..<<<...........>>>.<<.<..>.>>.<<<<....>>>>
..<<<<<.......>>>>>>>>>>>>....
Receptor-Ligand Complex Structure
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PDB4ari Structural Dynamics of the Aminoacylation and Proofreading Functional Cycle of Bacterial Leucyl-tRNA Synthetase
Resolution2.08 Å
Binding residue
(original residue number in PDB)		S227 Y246 T247 T248 R249 K300 N324 F325 V326 L327 Y330 V335 M336 D342 R344 R416 K598 F648 E657 S661 K671 R672 R719 A727 M730 E731 N734 K738 L801 R811 L834 I848 L854 N856
Binding residue
(residue number reindexed from 1)		S198 Y217 T218 T219 R220 K261 N285 F286 V287 L288 Y291 V296 M297 D303 R305 R377 K559 F609 E618 S622 K632 R633 R680 A688 M691 E692 N695 K699 L762 R772 L795 I809 L815 N817
Enzymatic activity
Catalytic site (original residue number in PDB) L41 H49 H52 D80 F493 H533 F543 K619 K622
Catalytic site (residue number reindexed from 1) L42 H50 H53 D81 F454 H494 F504 K580 K583
Enzyme Commision number 6.1.1.4: leucine--tRNA ligase.
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004823 leucine-tRNA ligase activity
GO:0005515 protein binding
GO:0005524 ATP binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006429 leucyl-tRNA aminoacylation
GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4ari, PDBe:4ari, PDBj:4ari
PDBsum4ari
PubMed22683997
UniProtP07813|SYL_ECOLI Leucine--tRNA ligase (Gene Name=leuS)

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