Structure of PDB 3u2q Chain A Binding Site BS01

Receptor Information

>3u2q Chain A (length=385) Species: 83333 (Escherichia coli K-12)

ERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAARAFDQIDNAPEE
KATINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAAT
DGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLS
QYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDK
PFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGV
EMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEV
YILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG

Ligand information

>3u2q Chain B (length=12) Species: 35762 (Planobispora rosea)

SCNCVCGFCCSC

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3u2q Discovery of LFF571: an investigational agent for Clostridium difficile infection.
• Resolution: 2.7 Å
• Binding residue (original residue number in PDB): E215 D216 F218 T228 G229 T256 E259 F261 R262 N273 V274 G275 L277
• Binding residue (residue number reindexed from 1): E207 D208 F210 T220 G221 T248 E251 F253 R254 N265 V266 G267 L269

Enzymatic activity

• Catalytic site (original residue number in PDB): D21 K24 T25 T61 H84
• Catalytic site (residue number reindexed from 1): D16 K19 T20 T53 H76

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0032045 guanyl-nucleotide exchange factor complex

External links

• PDB: RCSB:3u2q, PDBe:3u2q, PDBj:3u2q
• PDBsum: 3u2q
• PubMed: 22315981
• UniProt: P0CE47|EFTU1_ECOLI Elongation factor Tu 1 (Gene Name=tufA)