Structure of PDB 3t3o Chain A Binding Site BS01

Receptor Information

>3t3o Chain A (length=553) Species: 262724 (Thermus thermophilus HB27)

SQGGPQDHVEIIPLGGMGEIGKNITVFRFRDEIFVLDGGLAFPEEGMPGV
DLLIPRVDYLIEHRHKIKAWVLTHGAEDHIGGLPFLLPMIFGKESPVPIY
GARLTLGLLRGKLEEFGLRPGAFNLKEISPDDRIQVGRYFTLDLFRMTHS
IPDNSGVVIRTPIGTIVHTGDFKLDPTPIDGKVSHLAKVAQAGAEGVLLL
IADATNAERPGYTPSEMEIAKELDRVIGRAPGRVFVTTFASHIHRIQSVI
WAAEKYGRKVAMEGRSMLKFSRIALELGYLKVKDRLYTLEEVKDLPDHQV
LILATGSQGQPMSVLHRLAFEGHAKMAIKPGDTVILSSSPIPGNEEAVNR
VINRLYALGAYVLYPPTYKVHASGHASQEELKLILNLTTPRFFLPWHGEV
RHQMNFKWLAESMSRPPEKTLIGENGAVYRLTRETFEKVGEVPHGVLYVD
GLGVGDITEEILADRRHMAEEGLVVITALAGEDPVVEVVSRGFVKAGERL
LGEVRRMALEALKNGVREKKPLERIRDDIYYPVKKFLKKATGRDPMILPV
VIE

Ligand information

>3t3o Chain B (length=5)

cuggu

.....

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3t3o Molecular Basis for the Recognition and Cleavage of RNA by the Bifunctional 5'-3' Exo/Endoribonuclease RNase J.
• Resolution: 2.5 Å
• Binding residue (original residue number in PDB): I21 F43 L53 D79 F240 A241 H243 G265 R266 S267 T306 Q309 Q311 M313 R318 I342 N345 G375 H398
• Binding residue (residue number reindexed from 1): I20 F42 L52 D78 F239 A240 H242 G264 R265 S266 T305 Q308 Q310 M312 R317 I341 N344 G374 H397

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3t3o, PDBe:3t3o, PDBj:3t3o
• PDBsum: 3t3o
• PubMed: 21893286
• UniProt: Q72JJ7|RNJ_THET2 Ribonuclease J (Gene Name=rnj)