Structure of PDB 3t3n Chain A Binding Site BS01

Receptor Information

>3t3n Chain A (length=550) Species: 262724 (Thermus thermophilus HB27)

GPQDHVEIIPLGGMGEIGKNITVFRFRDEIFVLDGGLAFPEEGMPGVDLL
IPRVDYLIEHRHKIKAWVLTHGAEDHIGGLPFLLPMIFGKESPVPIYGAR
LTLGLLRGKLEEFGLRPGAFNLKEISPDDRIQVGRYFTLDLFRMTHSIPD
NSGVVIRTPIGTIVHTGDFKLDPTPIDGKVSHLAKVAQAGAEGVLLLIAD
ATNAERPGYTPSEMEIAKELDRVIGRAPGRVFVTTFASHIHRIQSVIWAA
EKYGRKVAMEGRSMLKFSRIALELGYLKVKDRLYTLEEVKDLPDHQVLIL
ATGSQGQPMSVLHRLAFEGHAKMAIKPGDTVILSSSPIPGNEEAVNRVIN
RLYALGAYVLYPPTYKVHASGHASQEELKLILNLTTPRFFLPWHGEVRHQ
MNFKWLAESMSRPPEKTLIGENGAVYRLTRETFEKVGEVPHGVLYVDGLG
VGDITEEILADRRHMAEEGLVVITALAGEDPVVEVVSRGFVKAGERLLGE
VRRMALEALKNGVREKKPLERIRDDIYYPVKKFLKKATGRDPMILPVVIE

Ligand information

>3t3n Chain B (length=6)

uuccgu

......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3t3n Molecular Basis for the Recognition and Cleavage of RNA by the Bifunctional 5'-3' Exo/Endoribonuclease RNase J.
• Resolution: 3.09 Å
• Binding residue (original residue number in PDB): I21 F43 D52 D79 F240 A241 R266 S267 T306 Q311 R318 I342 H372 S374 G375 H376
• Binding residue (residue number reindexed from 1): I17 F39 D48 D75 F236 A237 R262 S263 T302 Q307 R314 I338 H368 S370 G371 H372

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0004534 5'-3' RNA exonuclease activity
• GO:0008270 zinc ion binding
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006364 rRNA processing
• GO:0006396 RNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3t3n, PDBe:3t3n, PDBj:3t3n
• PDBsum: 3t3n
• PubMed: 21893286
• UniProt: Q72JJ7|RNJ_THET2 Ribonuclease J (Gene Name=rnj)