Structure of PDB 3rii Chain A Binding Site BS01

Receptor Information

>3rii Chain A (length=212) Species: 9606 (Homo sapiens)

GEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFEKLKPVHGLIFL
FKWQPGEEPAGSVVQDSRLDTIFFAKQVINNASATQAIVSVLLNCTHQDV
HLGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVHNSFARQAKEEDAFHF
VSYVPVNGRLYELDGLREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGE
IRFNLMAIVSDR

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 3rii Chain A Residue 230

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3rii Crystal structure of the catalytic domain of UCHL5, a proteasome-associated human deubiquitinating enzyme, reveals an unproductive form of the enzyme.
• Resolution: 2.0008 Å
• Binding residue (original residue number in PDB): E109 E113
• Binding residue (residue number reindexed from 1): E104 E108
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): Q82 S88 H164 D179
• Catalytic site (residue number reindexed from 1): Q77 S83 H149 D164
• Enzyme Commision number: 3.4.19.12: ubiquitinyl hydrolase 1.

Gene Ontology

Molecular Function

• GO:0004843 cysteine-type deubiquitinase activity
• GO:0070628 proteasome binding

Biological Process

• GO:0006511 ubiquitin-dependent protein catabolic process
• GO:0016579 protein deubiquitination

Cellular Component

• GO:0031011 Ino80 complex

External links

• PDB: RCSB:3rii, PDBe:3rii, PDBj:3rii
• PDBsum: 3rii
• PubMed: 21995438
• UniProt: Q9Y5K5|UCHL5_HUMAN Ubiquitin carboxyl-terminal hydrolase isozyme L5 (Gene Name=UCHL5)