Structure of PDB 3oh6 Chain A Binding Site BS01

Receptor Information

>3oh6 Chain A (length=288) Species: 83333 (Escherichia coli K-12)

MADIGSEFYTLNWQPPYDWSWMLGFLAARAVSSVETVADSYYARSLAVGE
YRGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNG
ALGRLGAARPGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYG
ERLDDFPEYICFPTPQRLAAADPQALKALGMPLKRAEALIHLANAALEGT
LPMTIPGDVEQAMKTLQTFPGIGRWTANYFALRGWQAKDVFLPDDCLIKQ
RFPGMTPAQIRRYAERWKPWRSYALLHIWYTEGWQPDE

Ligand information

>3oh6 Chain B (length=11)

gcattcatgtc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3oh6 Structure of Escherichia coli AlkA in Complex with Undamaged DNA.
• Resolution: 2.894 Å
• Binding residue (original residue number in PDB): L125 P213 G214 G216 W218 T219
• Binding residue (residue number reindexed from 1): L132 P220 G221 G223 W225 T226

Enzymatic activity

• Catalytic site (original residue number in PDB): W218 Y222 D238
• Catalytic site (residue number reindexed from 1): W225 Y229 D245
• Enzyme Commision number: 3.2.2.21: DNA-3-methyladenine glycosylase II.

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003905 alkylbase DNA N-glycosylase activity
• GO:0005515 protein binding
• GO:0008725 DNA-3-methyladenine glycosylase activity
• GO:0016787 hydrolase activity
• GO:0032131 alkylated DNA binding
• GO:0043916 DNA-7-methylguanine glycosylase activity
• GO:0052821 DNA-7-methyladenine glycosylase activity
• GO:0052822 DNA-3-methylguanine glycosylase activity

Biological Process

• GO:0006281 DNA repair
• GO:0006284 base-excision repair
• GO:0006285 base-excision repair, AP site formation
• GO:0006307 DNA alkylation repair
• GO:0006950 response to stress
• GO:0006974 DNA damage response

Cellular Component

• GO:0005737 cytoplasm
• GO:0032993 protein-DNA complex

External links

• PDB: RCSB:3oh6, PDBe:3oh6, PDBj:3oh6
• PDBsum: 3oh6
• PubMed: 20843803
• UniProt: P04395|3MG2_ECOLI DNA-3-methyladenine glycosylase 2 (Gene Name=alkA)