Structure of PDB 2xrz Chain A Binding Site BS01

Receptor Information

>2xrz Chain A (length=450) Species: 192952 (Methanosarcina mazei Go1)

HMNPKRIRALKSGKQGDGPVVYWMSRDQRAEDNWALLFSRAIAKEANVPV
VVVFCLTDEFLEAGIRQYEFMLKGLQELEVSLSRKKIPSFFLRGDPGEKI
SRFVKDYNAGTLVTDFSPLRIKNQWIEKVISGISIPFFEVDAHNVVPCWE
ASQKHEYAAHTFRPKLYALLPEFLEEFPELEPNSVETLSDVLETGVKALL
PERALLKNKDPLFEPWHFEPGEKAAKKVMESFIADRLDSYGALRNDPTKN
MLSNLSPYLHFGQISSQRVVLEVEKAESNPGSKKAFLDEILIWKEISDNF
CYYNPGYDGFESFPSWAKESLNAHRNDVRSHIYTLEEFEAGKTHDPLWNA
SQMELLSTGKMHGYTRMYWAKKILEWSESPEKALEIAICLNDRYELDGRD
PNGYAGIAWSIGGVHDRAWGEREVTGKIRYMSYEGCKRKFDVKLYIEKYS

Ligand information

>2xrz Chain C (length=11)

cggctcgcgca

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2xrz Crystal Structures of an Archaeal Class II DNA Photolyase and its Complex with Uv-Damaged Duplex DNA.
• Resolution: 2.2 Å
• Binding residue (original residue number in PDB): A160 H161 R164 N257 E301 W305 Y376 M379 W421 R429 W431 R441 Y442 M443 S444 R450 K451
• Binding residue (residue number reindexed from 1): A159 H160 R163 N245 E289 W293 Y364 M367 W409 R417 W419 R429 Y430 M431 S432 R438 K439
• Binding affinity: PDBbind-CN: Kd=44nM

Enzymatic activity

• Catalytic site (original residue number in PDB): E301 W305 A335 G375
• Catalytic site (residue number reindexed from 1): E289 W293 A323 G363
• Enzyme Commision number: 4.1.99.3: deoxyribodipyrimidine photo-lyase.

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003677 DNA binding
• GO:0003904 deoxyribodipyrimidine photo-lyase activity
• GO:0016829 lyase activity

Biological Process

• GO:0000719 photoreactive repair
• GO:0006281 DNA repair

External links

• PDB: RCSB:2xrz, PDBe:2xrz, PDBj:2xrz
• PDBsum: 2xrz
• PubMed: 21892138
• UniProt: Q8PYK9