Structure of PDB 2w8l Chain A Binding Site BS01

Receptor Information

>2w8l Chain A (length=342) Species: 273057 (Saccharolobus solfataricus P2)

HMIVLFVDFDYFYAQVEEVLNPSLKGKPVVVCVFSGRFEDSGAVATANYE
ARKFGVKAGIPIVEAKKILPNAVYLPMRKEVYQQVSSRIMNLLREYSEKI
EIASIDEAYLDISDKVRDYREAYNLGLEIKNKILEKEKITVTVGISKNKV
FAKIAADMAKPNGIKVIDDEEVKRLIRELDIADVPGIGNITAEKLKKLGI
NKLVDTLSIEFDKLKGMIGEAKAKYLISLARDEYNEPIRTRVRKSIGRIV
TMKRNSRNLEEIKPYLFRAIEESYYKLDKRIPKAIHVVAVTEDLDIVSRG
RTFPHGISKETAYSESVKLLQKILEEDERKIRRIGVRFSKFI

Ligand information

>2w8l Chain P (length=14)

gggggaaggattcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2w8l Versatility of Y-Family Sulfolobus Solfataricus DNA Polymerase Dpo4 in Translesion Synthesis Past Bulky N2-Alkylguanine Adducts.
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): S103 E106 K152 P184 G185 G187 I189 T190 R240 V296 S297 R298 T301 I341
• Binding residue (residue number reindexed from 1): S104 E107 K153 P185 G186 G188 I190 T191 R241 V297 S298 R299 T302 I342
• Binding affinity: PDBbind-CN: Kd=29uM

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0042276 error-prone translesion synthesis

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2w8l, PDBe:2w8l, PDBj:2w8l
• PDBsum: 2w8l
• PubMed: 19059910
• UniProt: Q97W02|DPO4_SACS2 DNA polymerase IV (Gene Name=dbh)