Structure of PDB 2rhq Chain A Binding Site BS01

Receptor Information

>2rhq Chain A (length=270) Species: 1283 (Staphylococcus haemolyticus)

LMEKLNQQLAEETIDVTLPSRQISIGSKHPLTRTVEEIEDLFLGLGYEIV
DGYEVEQDYYNFEALNLPKSHPARDMQDSFYITDEILMRTHTSPVQARTM
EKRNGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGLVVDKNIKMSDLKG
TLELVAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCNVCKHT
GWIEILGAGMVHPNVLEMAGFDSNEYSGFAFGMGPDRIAMLKYGIEDIRY
FYTNDVRFLEQFKAVEDRGE

Ligand information

• Ligand ID: ZN
• InChI: InChI=1S/Zn/q+2
• InChIKey: PTFCDOFLOPIGGS-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Zn++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Zn+2]
• Formula: Zn
• Name: ZINC ION
• ChEMBL: CHEMBL1236970
• DrugBank: DB14532
• PDB chain: 2rhq Chain A Residue 1

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2rhq Rational protein engineering in action: the first crystal structure of a phenylalanine tRNA synthetase from Staphylococcus haemolyticus.
• Resolution: 2.2 Å
• Binding residue (original residue number in PDB): C267 C270 C275 C278
• Binding residue (residue number reindexed from 1): C186 C189 C194 C197
• Annotation score: 1

Enzymatic activity

• Catalytic site (original residue number in PDB): Q158 H172 R200 Q214 T257 A311
• Catalytic site (residue number reindexed from 1): Q77 H91 R119 Q133 T176 A230
• Enzyme Commision number: 6.1.1.20: phenylalanine--tRNA ligase.

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004826 phenylalanine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006432 phenylalanyl-tRNA aminoacylation
• GO:0043039 tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:2rhq, PDBe:2rhq, PDBj:2rhq
• PDBsum: 2rhq
• PubMed: 18086534
• UniProt: Q4L5E3|SYFA_STAHJ Phenylalanine--tRNA ligase alpha subunit (Gene Name=pheS)