Structure of PDB 2phk Chain A Binding Site BS01

Receptor Information

>2phk Chain A (length=277) Species: 9986 (Oryctolagus cuniculus)

GFYENYEPKEILGRGVSSVVRRCIHKPTCKEYAVKIIDVTGGGSFSAEEV
QELREATLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKKGELFD
YLTEKVTLSEKETRKIMRALLEVICALHKLNIVHRDLKPENILLDDDMNI
KLTDFGFSCQLDPGEKLREVCGTPSYLAPEIIECSMNDNHPGYGKEVDMW
STGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDL
VSRFLVVQPQKRYTAEEALAHPFFQQY

Ligand information

>2phk Chain B (length=7)

RQMSFRL

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2phk The crystal structure of a phosphorylase kinase peptide substrate complex: kinase substrate recognition.
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): E110 F170 E182 V183 C184 G185 T186 P187 S188
• Binding residue (residue number reindexed from 1): E97 F157 E169 V170 C171 G172 T173 P174 S175

Enzymatic activity

• Catalytic site (original residue number in PDB): D149 K151 E153 N154 D167 T186
• Catalytic site (residue number reindexed from 1): D136 K138 E140 N141 D154 T173
• Enzyme Commision number: 2.7.11.1: non-specific serine/threonine protein kinase.
  2.7.11.19: phosphorylase kinase.
  2.7.11.26: [tau protein] kinase.

Gene Ontology

Molecular Function

• GO:0004672 protein kinase activity
• GO:0004689 phosphorylase kinase activity
• GO:0005516 calmodulin binding
• GO:0005524 ATP binding

Biological Process

• GO:0005977 glycogen metabolic process
• GO:0006468 protein phosphorylation

Cellular Component

• GO:0005964 phosphorylase kinase complex

External links

• PDB: RCSB:2phk, PDBe:2phk, PDBj:2phk
• PDBsum: 2phk
• PubMed: 9362479
• UniProt: P00518|PHKG1_RABIT Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform (Gene Name=PHKG1)