Structure of PDB 2ibt Chain A Binding Site BS01

Receptor Information

>2ibt Chain A (length=393) Species: 271 (Thermus aquaticus)

VETPPEVVDFMVSLAEAPRGGRVLEPACAHGPFLRAFREAHGTAYRFVGV
EIDPKALDLPPWAEGILADFLLWEPGEAFDLILGNPPYGIVGEASKYPIH
VFKAVKDLYKKAFSTWKGKYNLYGAFLEKAVRLLKPGGVLVFVVPATWLV
LEDFALLREFLAREGKTSVYYLGEVFPQKKVSAVVIRFQKSGKGLSLWDT
QESESGFTPILWAEYPHWEGEIIRFETEETRKLEISGMPLGDLFHIRFAA
RSPEFKKHPAVRKEPGPGLVPVLTGRNLKPGWVDYEKNHSGLWMPKERAK
ELRDFYATPHLVVAHTKGTRVVAAWDERAYPWREEFHLLPKEGVRLDPSS
LVQWLNSEAMQKHVRTLYRDFVPHLTLRMLERLPVRREYGFHT

Ligand information

>2ibt Chain B (length=10)

gttcggtgtc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2ibt 2-Aminopurine Flipped into the Active Site of the Adenine-Specific DNA Methyltransferase M.TaqI: Crystal Structures and Time-Resolved Fluorescence
• Resolution: 1.7 Å
• Binding residue (original residue number in PDB): V21 N105 Y108 K139 T167 L171 E172 D173 F196 K199 K200 F268 R271 R323 H394
• Binding residue (residue number reindexed from 1): V1 N85 Y88 K119 T147 L151 E152 D153 F176 K179 K180 F248 R251 R303 H374
• Binding affinity: PDBbind-CN: Kd=0.5uM

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:2ibt, PDBe:2ibt, PDBj:2ibt
• PDBsum: 2ibt
• PubMed: 17455934
• UniProt: P14385|MTTA_THEAQ Type II methyltransferase M.TaqI (Gene Name=taqIM)