Structure of PDB 2i82 Chain A Binding Site BS01

Receptor Information
>2i82 Chain A (length=217) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQR
DYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARV
VLKPITGRSHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTI
THPAYGNSMTFKAPADF
Ligand information
>2i82 Chain E (length=21) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gaggggauugaaaauccccuc
<<<<<<.........>>>>>>
Receptor-Ligand Complex Structure
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PDB2i82 Crystal structure of pseudouridine synthase RluA: indirect sequence readout through protein-induced RNA structure
Resolution2.05 Å
Binding residue
(original residue number in PDB)		M3 G37 R38 H61 R62 D64 M65 K77 E80 R81 K84 R88 K94 W119 R122 P123 Q125 K133 G159 R160 S161 H162 R179 F180
Binding residue
(residue number reindexed from 1)		M1 G35 R36 H59 R60 D62 M63 K75 E78 R79 K82 R86 K92 W117 R120 P121 Q123 K131 G157 R158 S159 H160 R177 F178
Enzymatic activity
Enzyme Commision number 5.4.99.28: tRNA pseudouridine(32) synthase.
5.4.99.29: 23S rRNA pseudouridine(746) synthase.
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0009982 pseudouridine synthase activity
GO:0016853 isomerase activity
GO:0106029 tRNA pseudouridine synthase activity
GO:0120159 rRNA pseudouridine synthase activity
GO:0140098 catalytic activity, acting on RNA
GO:0160142 23S rRNA pseudouridine(746) synthase activity
GO:0160151 tRNA pseudouridine(32) synthase activity
Biological Process
GO:0000455 enzyme-directed rRNA pseudouridine synthesis
GO:0001522 pseudouridine synthesis
GO:0006364 rRNA processing
GO:0006396 RNA processing
GO:0008033 tRNA processing
GO:0009451 RNA modification
GO:0031118 rRNA pseudouridine synthesis
GO:0031119 tRNA pseudouridine synthesis

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Molecular Function
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Biological Process
External links
PDB RCSB:2i82, PDBe:2i82, PDBj:2i82
PDBsum2i82
PubMed17188032
UniProtP0AA37|RLUA_ECOLI Dual-specificity RNA pseudouridine synthase RluA (Gene Name=rluA)

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