Structure of PDB 2hhs Chain A Binding Site BS01

Receptor Information

>2hhs Chain A (length=580) Species: 1422 (Geobacillus stearothermophilus)

KKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PDTKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>2hhs Chain B (length=13)

catgcgagtcagg

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2hhs The structural basis for the mutagenicity of O6-methyl-guanine lesions.
• Resolution: 1.8 Å
• Binding residue (original residue number in PDB): A433 T550 K551 T552 T556 S557 A558 R578 K582 Y587 R615 Q624 N625 I626 P627 R629 Y714 V828 H829 D830
• Binding residue (residue number reindexed from 1): A137 T254 K255 T256 T260 S261 A262 R282 K286 Y291 R319 Q328 N329 I330 P331 R333 Y418 V532 H533 D534

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:2hhs, PDBe:2hhs, PDBj:2hhs
• PDBsum: 2hhs
• PubMed: 17179038
• UniProt: Q45458