Structure of PDB 2gie Chain A Binding Site BS01

Receptor Information
>2gie Chain A (length=257) Species: 727 (Haemophilus influenzae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SFIKPIYQDINSILIGQKVKRPKSGTLSGHAAGEPFEKLVYKFLKENLSD
LTFKQYEYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSI
ENLFEEKQNDTADILLVKDQFYELLDVKTRNISKSAQAPNIISAYKLAQT
CAKMIDNKEFDLFDINYLEVDWELNGEDLVCVSTSFAELFKSEPSELYIN
WAAAMQIQFHVRDLDQGFNGTREEWAKSYLKHFVTQAEQRAISMIDKFVK
PFKKYIL
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB2gie Alteration of Sequence Specificity of the Type II Restriction Endonuclease HincII through an Indirect Readout Mechanism.
Resolution2.6 Å
Binding residue
(original residue number in PDB)		H73 Y77 S90 K93 Q109 Q138 Y199 N201 A204 R241 K248
Binding residue
(residue number reindexed from 1)		H72 Y76 S89 K92 Q108 Q137 Y198 N200 A203 R240 K247
Binding affinityPDBbind-CN: Kd=0.58nM
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2gie, PDBe:2gie, PDBj:2gie
PDBsum2gie
PubMed16675462
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

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