Structure of PDB 2aae Chain A Binding Site BS01

Receptor Information

>2aae Chain A (length=104) Species: 5062 (Aspergillus oryzae)

ACDYTCGSNCYSSSDVSTAQAAGYKLHEDGETVGSNSYPKKYNNYEGFDF
SVSSPYYEWPILSSGDVYSGGSPGADRVVFNENNQLAGVITHTGASGNNF
VECT

Ligand information

• Ligand ID: PO4
• InChI: InChI=1S/H3O4P/c1-5(2,3)4/h(H3,1,2,3,4)/p-3
• InChIKey: NBIIXXVUZAFLBC-UHFFFAOYSA-K
• SMILES:
  CACTVS 3.341: [O-][P]([O-])([O-])=O
  ACDLabs 10.04: [O-]P([O-])([O-])=O
  OpenEye OEToolkits 1.5.0: [O-]P(=O)([O-])[O-]
• Formula: O4 P
• Name: PHOSPHATE ION
• DrugBank: DB14523
• PDB chain: 2aae Chain A Residue 106

Receptor-Ligand Complex Structure

Structure summary

• PDB: 2aae Role of histidine-40 in ribonuclease T1 catalysis: three-dimensionalstructures of the partially active His40Lys mutant.
• Resolution: 1.8 Å
• Binding residue (original residue number in PDB): Y38 K40 E58 R77 H92 F100
• Binding residue (residue number reindexed from 1): Y38 K40 E58 R77 H92 F100
• Annotation score: 3

Enzymatic activity

• Catalytic site (original residue number in PDB): Y38 K40 E58 D76 R77 T91 H92 F100
• Catalytic site (residue number reindexed from 1): Y38 K40 E58 D76 R77 T91 H92 F100
• Enzyme Commision number: 4.6.1.24: ribonuclease T1.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0016829 lyase activity
• GO:0046589 ribonuclease T1 activity

Biological Process

• GO:0008150 biological_process

Cellular Component

• GO:0001411 hyphal tip
• GO:0030428 cell septum

External links

• PDB: RCSB:2aae, PDBe:2aae, PDBj:2aae
• PDBsum: 2aae
• PubMed: 1445870
• UniProt: P00651|RNT1_ASPOR Guanyl-specific ribonuclease T1 (Gene Name=rntA)