Structure of PDB 1x3z Chain A Binding Site BS01

Receptor Information

>1x3z Chain A (length=320) Species: 4932 (Saccharomyces cerevisiae)

NNIDFDSIAKMLLIKYKDFILSKFKKAAPVENIRFQNLVHTNQFAQGVLG
QSQHLCTVYDNPSWHSIVLETLDLDLIYKNVDKEFAKDGHAEGENIYTDY
LVKELLRYFKQDFFKWCNKPDCNHCGQNTSENMTPLGSQGPNGEESKFNC
GTVEIYKCNRCGNITRFPRYNDPIKLLETRKGRCGEWCNLFTLILKSFGL
DVRYVWNREDHVWCEYFSNFLNRWVHVDSCEQSFDQPYIYSINWNKKMSY
CIAFGKDGVVDVSKRYILQNELPRDQIKEEDLKFLCQFITKRLRYSLNDD
EIYQLACRDEQEQIELIRGK

Ligand information

>1x3z Chain I (length=3)

VAD

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1x3z Structure of a peptide:N-glycanase-Rad23 complex: insight into the deglycosylation for denatured glycoproteins.
• Resolution: 2.8 Å
• Binding residue (original residue number in PDB): R176 R190 C191 G192 D217
• Binding residue (residue number reindexed from 1): R169 R183 C184 G185 D210

Enzymatic activity

• Enzyme Commision number: 3.5.1.52: peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.

Gene Ontology

Molecular Function

• GO:0000224 peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006515 protein quality control for misfolded or incompletely synthesized proteins
• GO:0036503 ERAD pathway
• GO:0097466 ubiquitin-dependent glycoprotein ERAD pathway

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005829 cytosol
• GO:0120125 PNGase complex

External links

• PDB: RCSB:1x3z, PDBe:1x3z, PDBj:1x3z
• PDBsum: 1x3z
• PubMed: 15964983
• UniProt: Q02890|PNG1_YEAST Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase (Gene Name=PNG1)