Structure of PDB 1wse Chain A Binding Site BS01

Receptor Information

>1wse Chain A (length=155) Species: 562 (Escherichia coli)

MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTNNRMALM
AAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKARGWKTADKKPVKN
VDLWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTG
YQVEV

Ligand information

• Ligand ID: MN
• InChI: InChI=1S/Mn/q+2
• InChIKey: WAEMQWOKJMHJLA-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mn+2]
  CACTVS 3.341: [Mn++]
• Formula: Mn
• Name: MANGANESE (II) ION
• DrugBank: DB06757
• PDB chain: 1wse Chain A Residue 1001

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1wse Identification of Single Mn(2+) Binding Sites Required for Activation of the Mutant Proteins of E.coli RNase HI at Glu48 and/or Asp134 by X-ray Crystallography
• Resolution: 2.3 Å
• Binding residue (original residue number in PDB): D10 D134
• Binding residue (residue number reindexed from 1): D10 D134
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): D10 G11 A48 D70 H124 D134
• Catalytic site (residue number reindexed from 1): D10 G11 A48 D70 H124 D134
• Enzyme Commision number: 3.1.26.4: ribonuclease H.

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003676 nucleic acid binding
• GO:0004519 endonuclease activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity
• GO:0005515 protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006401 RNA catabolic process
• GO:0043137 DNA replication, removal of RNA primer

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1wse, PDBe:1wse, PDBj:1wse
• PDBsum: 1wse
• PubMed: 15644213
• UniProt: P0A7Y4|RNH_ECOLI Ribonuclease HI (Gene Name=rnhA)