Structure of PDB 1uaa Chain A Binding Site BS01

Receptor Information
>1uaa Chain A (length=636) Species: 83333 (Escherichia coli K-12) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
RLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIA
AVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGM
KANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQ
AAASAIGERDRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQANEEVR
KRWQNKIRYLLVDEYQDTNTSQYELVKLLVGSRARFTVVGDDDQSIYSWR
GARPQNLVLLSQDFPALKVIKLEQNYRSSGRILKAANILIANNPHVFEKR
LFSELGYGAELKVLSANNEEHEAERVTGELIAHHFVNKTQYKDYAILYRG
NHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRVLTNPDDDSA
FLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGYE
ALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMR
MKNVNQLFSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMEREEELDQVQL
MTLHASKGLEFPYVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQ
KELTFTLCKERRQYGELVRPEPSRFLLELPQDDLIW
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB1uaa Major domain swiveling revealed by the crystal structures of complexes of E. coli Rep helicase bound to single-stranded DNA and ADP.
Resolution3.0 Å
Binding residue
(original residue number in PDB)		T56 N57 H85 Q133 F183 W250 R251 R350 G351 N352 H353 R356 T556 H558 H580 S582
Binding residue
(residue number reindexed from 1)		T55 N56 H84 Q132 F182 W249 R250 R349 G350 N351 H352 R355 T552 H554 H576 S578
Enzymatic activity
Catalytic site (original residue number in PDB) T29 D214 Q245
Catalytic site (residue number reindexed from 1) T28 D213 Q244
Enzyme Commision number 5.6.2.4: DNA 3'-5' helicase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003678 DNA helicase activity
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016853 isomerase activity
GO:0016887 ATP hydrolysis activity
GO:0042803 protein homodimerization activity
GO:0043138 3'-5' DNA helicase activity
Biological Process
GO:0000725 recombinational repair
GO:0006260 DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006269 DNA replication, synthesis of primer
GO:0009314 response to radiation
GO:0031297 replication fork processing
GO:0044787 bacterial-type DNA replication
Cellular Component
GO:0005829 cytosol
GO:0043600 cytoplasmic replisome
GO:1990077 primosome complex
GO:1990160 DnaB-DnaC-Rep-PriC complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1uaa, PDBe:1uaa, PDBj:1uaa
PDBsum1uaa
PubMed9288744
UniProtP09980|REP_ECOLI ATP-dependent DNA helicase Rep (Gene Name=rep)

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