Structure of PDB 1tw8 Chain A Binding Site BS01

Receptor Information
>1tw8 Chain A (length=257) Species: 727 (Haemophilus influenzae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SFIKPIYQDINSILIGQKVKRPKSGTLSGHAAGEPFEKLVYKFLKENLSD
LTFKQYEYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSI
ENLFEEKQNDTADILLVKDQFYELLDVKTRNISKSAQAPNIISAYKLAQT
CAKMIDNKEFDLFDINYLEVDWELNGEDLVCVSTSFAELFKSEPSELYIN
WAAAMQIQFHVRDLDQGFNGTREEWAKSYLKHFVTQAEQRAISMIDKFVK
PFKKYIL
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB1tw8 Ca2+ binding in the active site of HincII: implications for the catalytic mechanism
Resolution2.8 Å
Binding residue
(original residue number in PDB)		H31 Y77 G92 K93 Q109 Q138 Y199 N201 A204 K248
Binding residue
(residue number reindexed from 1)		H30 Y76 G91 K92 Q108 Q137 Y198 N200 A203 K247
Binding affinityPDBbind-CN: Kd=1.14nM
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1tw8, PDBe:1tw8, PDBj:1tw8
PDBsum1tw8
PubMed15491133
UniProtP17743|T2C2_HAEIF Type II restriction enzyme HincII (Gene Name=hincIIR)

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