Structure of PDB 1t9i Chain A Binding Site BS01
Receptor Information
>1t9i Chain A (length=153) Species:
3055
(Chlamydomonas reinhardtii) [
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NTKYNKEFLLYLAGFVDGNGSIIAQIKPNQSYKFKHQLSLTFQVTQKTQR
RWFLDKLVDEIGVGYVRDRGSVSDYILSEIKPLHNFLTQLQPFLKLKQKQ
ANLVLKIIEQLPSAKESPDKFLEVCTWVDQIAALNDSKTRKTTSETVRAV
LDS
Ligand information
>1t9i Chain C (length=24) [
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gcaaaacgtcgtgagacagtttcg
Receptor-Ligand Complex Structure
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PDB
1t9i
Metal-Dependent DNA Cleavage Mechanism of the I-CreI LAGLIDADG Homing Endonuclease.
Resolution
1.6 Å
Binding residue
(original residue number in PDB)
N20 S22 Q26 N30 Q44 T46 Q47 K48 R51 R70 V73 N136 D137 S138 R141 K142
Binding residue
(residue number reindexed from 1)
N19 S21 Q25 N29 Q43 T45 Q46 K47 R50 R69 V72 N135 D136 S137 R140 K141
Binding affinity
PDBbind-CN
: Kd=1.8nM
Enzymatic activity
Catalytic site (original residue number in PDB)
G19 N20
Catalytic site (residue number reindexed from 1)
G18 N19
Enzyme Commision number
3.1.-.-
Gene Ontology
Molecular Function
GO:0004519
endonuclease activity
GO:0042802
identical protein binding
GO:0046872
metal ion binding
Biological Process
GO:0006314
intron homing
Cellular Component
GO:0009507
chloroplast
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Cellular Component
External links
PDB
RCSB:1t9i
,
PDBe:1t9i
,
PDBj:1t9i
PDBsum
1t9i
PubMed
15518550
UniProt
P05725
|DNE1_CHLRE DNA endonuclease I-CreI
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