Structure of PDB 1t8i Chain A Binding Site BS01

Receptor Information

>1t8i Chain A (length=565) Species: 9606 (Homo sapiens)

QKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLS
PKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKC
DFTQMSQYFKAQTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERI
ANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHK
WKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRL
KKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETAD
TVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKN
LQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQ
QLKELTAPDENIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTK
IDAKKEQLADARRDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMK
LEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQRE
KFAWAIDMADEDYEF

Ligand information

>1t8i Chain B (length=10)

aaaaagactt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1t8i Structures of three classes of anticancer agents bound to the human topoisomerase I-DNA covalent complex
• Resolution: 3.0 Å
• Binding residue (original residue number in PDB): V410 T411 W412 I424 Y426 M428 K443 K532 K587 N722 Y723
• Binding residue (residue number reindexed from 1): V210 T211 W212 I224 Y226 M228 K243 K332 K387 N522 Y523
• Binding affinity: PDBbind-CN: IC50=0.3uM

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1t8i, PDBe:1t8i, PDBj:1t8i
• PDBsum: 1t8i
• PubMed: 15801827
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)