Structure of PDB 1t0f Chain A Binding Site BS01

Receptor Information

>1t0f Chain A (length=264) Species: 562 (Escherichia coli)

NSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRSHRIYSHKTGR
VHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHPVI
RGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQ
QKQIPWFIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQE
KGDENIINVCKQVDIAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKC
ADLCISQVVNMEEL

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 1t0f Chain A Residue 1010

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1t0f The carboxy-terminal portion of TnsC activates the Tn7 transposase through a specific interaction with TnsA.
• Resolution: 1.85 Å
• Binding residue (original residue number in PDB): D114 Q130 V131
• Binding residue (residue number reindexed from 1): D110 Q126 V127
• Annotation score: 4

Enzymatic activity

• Enzyme Commision number: 3.1.21.-

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0004803 transposase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006310 DNA recombination
• GO:0006313 DNA transposition
• GO:0032196 transposition

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1t0f, PDBe:1t0f, PDBj:1t0f
• PDBsum: 1t0f
• PubMed: 15257292
• UniProt: P13988|TNSA_ECOLX Transposon Tn7 transposition protein TnsA (Gene Name=tnsA)