Structure of PDB 1qum Chain A Binding Site BS01

Receptor Information

>1qum Chain A (length=279) Species: 562 (Escherichia coli)

MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPLTTQTID
EFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQ
LGLSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQ
GSNLGFKFEHLAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTF
ADFARTVGFKYLRGMHLNDAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQD
DRFDGIPLILETINPDIWAEEIAWLKAQQ

Ligand information

>1qum Chain B (length=6)

gcgtcc

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1qum Structure of the DNA repair enzyme endonuclease IV and its DNA complex: double-nucleotide flipping at abasic sites and three-metal-ion catalysis.
• Resolution: 1.55 Å
• Binding residue (original residue number in PDB): R37 Y72 D229 R230 H231
• Binding residue (residue number reindexed from 1): R37 Y72 D229 R230 H231

Enzymatic activity

• Catalytic site (original residue number in PDB): R37 H69 Y72 H109 E145 D179 H182 H216 D229 H231 E261
• Catalytic site (residue number reindexed from 1): R37 H69 Y72 H109 E145 D179 H182 H216 D229 H231 E261
• Enzyme Commision number: 3.1.21.2: deoxyribonuclease IV.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity
• GO:0004519 endonuclease activity
• GO:0008081 phosphoric diester hydrolase activity
• GO:0008270 zinc ion binding
• GO:0008296 3'-5'-DNA exonuclease activity
• GO:0008833 deoxyribonuclease IV (phage-T4-induced) activity
• GO:0016791 phosphatase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006281 DNA repair
• GO:0006284 base-excision repair

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1qum, PDBe:1qum, PDBj:1qum
• PDBsum: 1qum
• PubMed: 10458614
• UniProt: P0A6C1|END4_ECOLI Endonuclease 4 (Gene Name=nfo)