Structure of PDB 1qu3 Chain A Binding Site BS01

Receptor Information
>1qu3 Chain A (length=880) Species: 1280 (Staphylococcus aureus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
DYEKTLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETFI
LHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPI
EQALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDP
YITLKPEYEAAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYH
DKRSASIYVAFNVKDDKGVVDADAKFIIWTTTPWTIPSNVAITVHPELKY
GQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELEWVVAQHP
FLDRESLVINGDHVTTDAGTGCVHTAPGHGEDDYIVGQQYELPVISPIDD
KGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRTK
KPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGEW
VISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAK
DLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADM
YLEGSDQYRGWFNSSITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLG
NVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQTSDDYRKIRNT
LRFMLGNINDFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFDY
LNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRSMQTVLYQILVD
MTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKWRTF
MNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQ
LFIVSQVKVVDKLDDQATAYEHGDIVIEHA
Ligand information
>1qu3 Chain T (length=75) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gggcuuguagcucaggugguuagagcgcaccccugauaagggugaggucg
gugguucaaguccacucaggcccac
<<<<<<<..<<<<.........>>>>.<<<<<.......>>>>>.....<
<<<<.......>>>>>>>>>>>>..
Receptor-Ligand Complex Structure
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PDB1qu3 Insights into editing from an ile-tRNA synthetase structure with tRNAile and mupirocin.
Resolution2.9 Å
Binding residue
(original residue number in PDB)		E4 M9 M16 R17 V315 D333 W436 R440 S556 D557 R560 F587 M589 G593 K594 K595 S624 T625 D626 D630 R632 E636 Q640 K647 N650 R653 F654 L702 Q706 Q709 N710 N713 S717 N718 K725 Y729 N809 R810 E813 R816 G822 K823
Binding residue
(residue number reindexed from 1)		E3 M8 M15 R16 V314 D332 W435 R439 S555 D556 R559 F586 M588 G592 K593 K594 S623 T624 D625 D629 R631 E635 Q639 K646 N649 R652 F653 L701 Q705 Q708 N709 N712 S716 N717 K724 Y728 N808 R809 E812 R815 G821 K822
Enzymatic activity
Catalytic site (original residue number in PDB) S186 S189 D397 T400 W528 K595 K598
Catalytic site (residue number reindexed from 1) S185 S188 D396 T399 W527 K594 K597
Enzyme Commision number 6.1.1.5: isoleucine--tRNA ligase.
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation
GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1qu3, PDBe:1qu3, PDBj:1qu3
PDBsum1qu3
PubMed10446055
UniProtP41972|SYI1_STAAU Isoleucine--tRNA ligase (Gene Name=ileS)

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