Structure of PDB 1qp7 Chain A Binding Site BS01

Receptor Information

>1qp7 Chain A (length=338) Species: 562 (Escherichia coli)

TIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELHYSPSAVAR
SLAVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKA
DFTDAVIDNAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMK
AMEEAMIKVPESWIVQGDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAM
GALCAADEMGLRVPQDVSLIGYDNVRNARYFTPALTTIHQPKDSLGETAF
NMLLDRIVNKREEPQSIEVHPRLIERRSVADGPFRDYR

Ligand information

>1qp7 Chain M (length=17)

tacgcaaccggttgcgt

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1qp7 The role of lysine 55 in determining the specificity of the purine repressor for its operators through minor groove interactions.
• Resolution: 2.9 Å
• Binding residue (original residue number in PDB): S14 T16 T17 R26 V28 A29 T32 L54
• Binding residue (residue number reindexed from 1): S12 T14 T15 R24 V26 A27 T30 L52
• Binding affinity: PDBbind-CN: Kd=780nM

Gene Ontology

Molecular Function

• GO:0000976 transcription cis-regulatory region binding
• GO:0001217 DNA-binding transcription repressor activity
• GO:0002057 guanine binding
• GO:0003677 DNA binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0005515 protein binding
• GO:0042803 protein homodimerization activity

Biological Process

• GO:0006164 purine nucleotide biosynthetic process
• GO:0006355 regulation of DNA-templated transcription
• GO:0045892 negative regulation of DNA-templated transcription
• GO:1900372 negative regulation of purine nucleotide biosynthetic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1qp7, PDBe:1qp7, PDBj:1qp7
• PDBsum: 1qp7
• PubMed: 10438625
• UniProt: P0ACP7|PURR_ECOLI HTH-type transcriptional repressor PurR (Gene Name=purR)