Structure of PDB 1nwx Chain A Binding Site BS01

Receptor Information

>1nwx Chain A (length=270) Species: 1299 (Deinococcus radiodurans)

KKYRPYTPSRRQMTTADFSGLTKKRPEKALTEALPKTGGRNNRGRITSRF
IGGGHKRLYRIIDFKRRDKSGVNAKVAAIEYDPNRSARIALLHYADGEKR
YILAPEGLTVGATVNAGPEAEPKLGNALPLRFVPVGAVVHALELVPGKGA
QLARSAGTSVQVQGKESDYVIVRLPSGELRRVHSECYATIGAVGNAEHKN
IVLGKAGRSRWLGRKPHQRGSAMNPVDHPHGGGEGRTGAGRVPVTPWGKP
TKGLKTRRKRKTSDRFIVTR

Ligand information

>1nwx Chain 0 (length=2766)

ggucaagauaguaaggguccacgguggaugcccuggcgcuggagccgaug
aaggacgcgauuaccugcgaaaagccccgacgagcuggagauacgcuuug
acucggggauguccgaauggggaaacccaccucguaagagguauccgcaa
ggaugggaacucagggaacugaaacaucucaguaccugaaggagaagaaa
gagaauucgauuccguuaguagcggcgagcgaacccggaucagcccaaau
ucaaccccucaagccgaaguggcuggaaagcuacaccucagaaggugaga
guccuguaggcgaacgagcgguugacuguaaggucguuguucgugaaacg
augacugaauccgcgcggaccaccgcgcaaggcuaaauacucccagugac
cgauagcgcauaguaccgugagggaaaggugaaaagaaccccgggagggg
agugaaagagaaccugaaaccguggacuuacaagcagucauggcaccuua
ugcguguuauggcgugccuauugaagcaugagccggcgacuuagaccuga
cgugcgagcuuaaguugaaaaacggaggcggagcgaaagcgaguccgaau
agggcggcauuaguacgucgggcuagacucgaaaccaggugagcuaagca
ugaccagguugaaacccccgugacagggggcggaggaccgaaccggugcc
ugcugaaacagucucggaugaguuguguuuaggagugaaaagcuaaccga
accuggagauagcuaguucuccccgaaauguauugagguacagccucgga
uguugaccauguccuguagagcacucacaaggcuaaaaccuuaugaaacu
ccgaaggggcacgcguuuaguccgggagugaggcugcgagagcuaacuuc
cguagccgagagggaaacaacccagaccaucagcuaaggucccuaaauga
ucgcucagugguuaaggaugugucgucgcauagacagccaggagguuggc
uuagaagcagccacccuucaaagagugcguaauagcucacuggucgagug
acgaugcgccgaaaaugaucggggcucaagugaucuaccgaagcuaugga
uucaacucgcgaagcgaguugucugguaggggagcguucaguccgcggag
aagccauaccggaaggagugguggagccgacugaagugcggaugccggca
ugaguaacgauaaaagaagugagaaucuucuucgccguaaggacaagggu
uccuggggaagggucguccgcccagggaaagucgggaccuaaggugaggc
cgaacggcgcagccgauggacagcaggucaagauuccugcaccgaucaug
uggagugauggagggacgcauuacgcuauccaaugccaagcuauggcuau
gcugguugguacgcucaagggcgaucgggucagaaaaucuaccggucaca
ugccucagacguaucgggagcuuccucggaagcgaaguuggaaacgcgac
ggugccaagaaaagcuucuaaacguugaaacaugauugcccguaccgcaa
accgacacagguguccgagugucaaugcacuaaggcgcgcgagagaaccc
ucguuaaggaacuuugcaaucucaccccguaacuucggaagaaggggucc
ccacgcuucgcguggggcgcagugaauaggcccaggcgacuguuuaccaa
aaucacagcacucugccaacacgaacaguggacguauagggugugacgcc
ugcccggugccggaaggucaaguggagcggugcaagcugcgaaaugaagc
cccggugaacggcggccguaacuauaacgguccuaagguagcgaaauucc
uugucggguaaguuccgaccugcacgaaaggcguaacgaucugggcgcug
ucucaacgagggacucggugaaauugaauuggcuguaaagaugcggccua
cccguagcaggacgaaaagaccccguggagcuuuacuauagucuggcauu
gggauucggguuucucugcguaggugggagaaacuggccggucgguggcc
acccugagaaacuuggauuucuaaccugaaaaaucacuuucggggaccgu
gcuuggcggguaguuugacuggggcggucgccucccaaaauguaacggag
gcgcccaaaggucaccucaagacgguuggaaaucgucuguagagcgcaaa
gguagaagguggcuugacugcgagacugacacgucgagcagggaggaaac
ucgggcuuagugaaccggugguaccguguggaagggccaucgaucaacgg
auaaaaguuaccccggggauaacaggcugaucucccccgagaguccauau
cggcggggagguuuggcaccucgaugucggcucgucgcauccuggggcug
aagaaggucccaaggguugggcuguucgcccauuaaagcggcacgcgagc
uggguucagaacgucgugagacaguucggucucuauccgcuacgggcgca
ggagaauugaggggaguugcuccuaguacgagaggaccggagugaacgga
ccgcuggucucccugcugucguaccaacggcacaugcaggguagcuaugu
ccggaacggauaaccgcugaaagcaucuaagcgggaagccagccccaaga
ugaguucucccacuguucagguaagacucccggaagaccaccggguuaag
aggccaggcgugcacgcauagcaauguguucagcggacuggugcucauca
gucgaggucuugacca

<<<<<<<<......<<<<<<<<<<<.....<<.<<<<<<<<<<<<<....
..<<<<<<<((..((>>>>.....<<<<<<...<<.))..))..>>....
..>>>>>>...>>>....<<<....>>><<<<<....>>>>>.<<<....
>>>.>>...<<<<<<.....<<....>>......>>>>>.>.........
<<....>>...<<<<......<<.....>>.....>>>>...<<<.....
<<<<<<.<<<..<<<...<<<<<......>>>>>((......<<<.....
))>>>...>>>....>>>.>>>>>>......<<<<<<<<.........>>
>>>>>>......<<<<<..((((>>>>>..>>>......>>.>>>>>>.>
>>.............<<....>>....>>.........<<<<....>>>>
..................>>>>>>>>>>>.......<<<<<<<<<<....
...>>>>>>>>>>...<.<........>.>.<<<<<<....<<<.<<<<<
<<<<<...<<...<<......>>...>>....<<....>>...<......
..>.........>>>>>>>>>>>>>..<<.<...<<<<<<....<<<<<<
<<<<..<<<.....<<<<<......>>>>>......>>>...<<<<.<.<
<<......>>>.>>>>>....>>>>>>>>>>..<<<.....>>>......
>>>>>>......>.>>..<<<<<<....<<<<<....>>>>><<<<<<<<
<...<<<<.<<<<<....<<....<<<.<<<<.<..>.>>>>.>>>...>
.>....>>>>>.>.>>>..>>>>>>>.>>..<<<<<<<.<<......>>.
>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....<<
<<<<...........<<..<<<<<<<<..........<<<..<<<...<<
.........>>...>>>.........<<......>>....>>>......>
>>>>>>>.>>..........)))......>>>>>>.>>>...>>>>>>>.
.<.<<<<<<.....>>>>>>>.......>>>>>>..<<<<<<<.<<....
..<<<<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><
......<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<..
.<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<
<<..>>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<
<...<<...<<<<....<<<<..<<<..<<<<<.<<<<.<<...[[....
>>>>>>.<.<<<<.<<.<<<..<<<<<<.............>>>>>>...
..>>>..>>>>>>>.....<<<<<....>>>>>....>>>>>...>>>..
.>>>>.........>>>>...>>......>>>>>>>>>>>>>..>>....
.<<......>>>>>>>.<<<<<....>>>>>..>>>>..<<<<....<<<
<<<<<.<<.........<.....<<<<<...............>>>>><<
<<<<<<...>>>>>>>>............<<<<<<<<<..<<........
...>>..<<<<<<<.....<<<.....>>>.......>>>>>>>.<<<<<
.<<<..<<<<<<<..<<<...<<...<<<<<....>>>>>...>>...>>
>>>>>>..>>>>><<<<<<.......>>>>>>........<.........
..<<<<<.......>>>>>.....>....>>>>>..>>.>>>>>>>..>.
.>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..
<<<<<<<<.<<<.........<<<<.<<<<<<..<<<<<.<<<.<<<<..
<<<<<<..<<<.<<<<.<....<<<<<.....<<<<<<..>>>>>>..>>
>>>>.>>>>.>>>..>>>>>>...<<<<<<........>>>>>>.....>
>>>.>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<....((
(.>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>
>>.>>))).......<<<<<<<.<<..))))..>>>>>>>>>........
.....>>>>..>>>>>>.......<<<<.<<<<<<<<.<.<<.......>
>..>>>>>>>>..>>>>.>.......<<<<<.<.<<<<..<<<<<<<<<.
.....>>>>>>>..>>.<<<<<.....>>>>>.....>>>>...>...>>
>.>>....<<<<<<<....>>>.>>>>........>>>>>>>>>>>....
<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<
<....<<<...<<<<<<<<<<<.]].>>>>>>...>>>>>...>>>..>>
>>>....<<....<<<<.............>>>>....>>..>>>>....
...>>>>>>>..<<<..>>>....<<<<<<<<<.......>>>>>....<
<<<<<<<.<<<....<<<<......>>>>...>>>..>>>>>.>>>...>
>>>....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1nwx Structural basis for the antibiotic activity of ketolides and azalides.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): R48 D85 G100 A153 Q154 L155 R157 G210 G234 G236 G238 A242
• Binding residue (residue number reindexed from 1): R45 D82 G97 A150 Q151 L152 R154 G207 G231 G233 G235 A239

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0016740 transferase activity
• GO:0019843 rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:1nwx, PDBe:1nwx, PDBj:1nwx
• PDBsum: 1nwx
• PubMed: 12623020
• UniProt: Q9RXJ9|RL2_DEIRA Large ribosomal subunit protein uL2 (Gene Name=rplB)