Structure of PDB 1l5u Chain A Binding Site BS01

Receptor Information

>1l5u Chain A (length=580) Species: 1422 (Geobacillus stearothermophilus)

AKMAFTLADRVTEEMLADKAALVVEVVEENYHDAPIVGIAVVNEHGRFFL
RPETALADPQFVAWLGDETKKKSMFDSKRAAVALKWKGIELCGVSFDLLL
AAYLLDPAQGVDDVAAAAKMKQYEAVRPDEAVYGKGAKRAVPDEPVLAEH
LVRKAAAIWELERPFLDELRRNEQDRLLVELEQPLSSILAEMEFAGVKVD
TKRLEQMGKELAEQLGTVEQRIYELAGQEFNINSPKQLGVILFEKLQLPV
LKKTKTGYSTSADVLEKLAPYHEIVENILHYRQLGKLQSTYIEGLLKVVR
PDTKKVHTIFNQALTQTGRLSSTEPNLQNIPIRLEEGRKIRQAFVPSESD
WLIFAADYSQIELRVLAHIAEDDNLMEAFRRDLDIHTKTAMDIFQVSEDE
VTPNMRRQAKAVNFGIVYGISDYGLAQNLNISRKEAAEFIERYFESFPGV
KRYMENIVQEAKQKGYVTTLLHRRRYLPDITSRNFNVRSFAERMAMNTPI
QGSAADIIKKAMIDLNARLKEERLQAHLLLQVHDELILEAPKEEMERLCR
LVPEVMEQAVTLRVPLKVDYHYGSTWYDAK

Ligand information

>1l5u Chain B (length=12)

gcgatcacgtac

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1l5u Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations
• Resolution: 1.95 Å
• Binding residue (original residue number in PDB): G432 A433 T550 S555 T556 S557 A558 R578 K582 R615 Q624 N625 I626 P627 I628 R629 V828 H829 D830
• Binding residue (residue number reindexed from 1): G136 A137 T254 S259 T260 S261 A262 R282 K286 R319 Q328 N329 I330 P331 I332 R333 V532 H533 D534

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0008408 3'-5' exonuclease activity

Biological Process

• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication

External links

• PDB: RCSB:1l5u, PDBe:1l5u, PDBj:1l5u
• PDBsum: 1l5u
• PubMed: 12649320
• UniProt: P52026|DPO1_GEOSE DNA polymerase I (Gene Name=polA)