Structure of PDB 1j2b Chain A Binding Site BS01

Receptor Information
>1j2b Chain A (length=576) Species: 53953 (Pyrococcus horikoshii) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MLKFEIKARDGAGRIGKLEVNGKKIETPAIMPVVNPKQMVVEPKELEKMG
FEIIITNSYIIYKDEELRRKALELGIHRMLDYNGIIEVDSGSFQLMKYGS
IEVSNREIIEFQHRIGVDIGTFLDIPTPPDAPREQAVKELEITLSRAREA
EEIKEIPMNATIQGSTYTDLRRYAARRLSSMNFEIHPIGGVVPLLESYRF
RDVVDIVISSKMALRPDRPVHLFGAGHPIVFALAVAMGVDLFDSASYALY
AKDDRYMTPEGTKRLDELDYFPCSCPVCSKYTPQELREMPKEERTRLLAL
HNLWVIKEEIKRVKQAIKEGELWRLVDERARSHPKLYSAYKRLLEHYTFL
EEFEPITKKSALFKISNESLRWPVVRRAKERAKSINERFGELVEHPIFGR
VSRYLSLTYPFAQSEAEDDFKIEKPTKEDAIKYVMAIAEYQFGEGASRAF
DDAKVELSKTGMPRQVKVNGKRLATVRADDGLLTLGIEGAKRLHRVLPYP
RMRVVVNKEAEPFARKGKDVFAKFVIFADPGIRPYDEVLVVNENDELLAT
GQALLSGREMIVFQYGRAVKVRKGVE
Ligand information
>1j2b Chain C (length=77) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gggcccguggucuaguuggucaugacgccgcccuuacgaggcggaggucc
gggguucaaguccccgcgggcccacca
<<<<<<.................<<<.<<<<<.......>>>>>..>>><
<<<<.......>>>>>.>>>>>>....
Receptor-Ligand Complex Structure
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PDB1j2b Alternative Tertiary Structure of tRNA for Recognition by a Posttranscriptional Modification Enzyme
Resolution3.3 Å
Binding residue
(original residue number in PDB)		A367 A418 Q419 E421 D424 D425 I428 K430 K465 T466 M468 R470 Q471 R478 T481 A484 T490 E515 F519 D525 F527 K529 F530
Binding residue
(residue number reindexed from 1)		A361 A412 Q413 E415 D418 D419 I422 K424 K459 T460 M462 R464 Q465 R472 T475 A478 T484 E509 F513 D519 F521 K523 F524
Enzymatic activity
Catalytic site (original residue number in PDB) D249 C279 C281 C284 H307
Catalytic site (residue number reindexed from 1) D243 C273 C275 C278 H301
Enzyme Commision number 2.4.2.48: tRNA-guanine(15) transglycosylase.
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0008270 zinc ion binding
GO:0016757 glycosyltransferase activity
GO:0016763 pentosyltransferase activity
GO:0046872 metal ion binding
Biological Process
GO:0002099 tRNA wobble guanine modification
GO:0006400 tRNA modification
GO:0008033 tRNA processing
GO:0008616 queuosine biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Biological Process
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Cellular Component
External links
PDB RCSB:1j2b, PDBe:1j2b, PDBj:1j2b
PDBsum1j2b
PubMed12732145
UniProtO58843|ATGT_PYRHO tRNA-guanine(15) transglycosylase (Gene Name=tgtA)

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