Structure of PDB 1ffy Chain A Binding Site BS01

Receptor Information
>1ffy Chain A (length=917) Species: 1280 (Staphylococcus aureus) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MDYEKTLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETF
ILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLP
IEQALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFND
PYITLKPEYEAAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEY
HDKRSASIYVAFNVKDDKGVVDADAKFIIWTTTPWTIPSNVAITVHPELK
YGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELEWVVAQH
PFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDDYIVGQQYELPVISPID
DKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRT
KKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREA
KDLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPAD
MYLEGSDQYRGWFNSSITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSL
GNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQTSDDYRKIRN
TLRFMLGNINDFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFD
YLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRSMQTVLYQILV
DMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKWRT
FMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALH
QLFIVSQVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVD
ELTHLCPRCQQVVKSLV
Ligand information
>1ffy Chain T (length=75) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
gggcuuguagcucaggugguuagagcgcaccccugauaagggugaggucg
gugguucaaguccacucaggcccac
<<<<<<<..<<<<.........>>>>.<<<<<.......>>>>>.....<
<<<<.......>>>>>>>>>>>>..
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB1ffy Insights into editing from an ile-tRNA synthetase structure with tRNAile and mupirocin.
Resolution2.2 Å
Binding residue
(original residue number in PDB)		E4 L7 M9 M16 R17 D333 W436 R440 N443 S556 D557 R560 F587 M589 G593 K595 S624 T625 D626 D630 R632 E636 Q640 K647 N650 R653 F654 L702 Q706 Q709 N710 N713 S717 N718 D722 K725 Y729 E813 R816 G822 K823 R888 W890
Binding residue
(residue number reindexed from 1)		E4 L7 M9 M16 R17 D333 W436 R440 N443 S556 D557 R560 F587 M589 G593 K595 S624 T625 D626 D630 R632 E636 Q640 K647 N650 R653 F654 L702 Q706 Q709 N710 N713 S717 N718 D722 K725 Y729 E813 R816 G822 K823 R888 W890
Enzymatic activity
Catalytic site (original residue number in PDB) S186 S189 D397 T400 W528 K595 K598
Catalytic site (residue number reindexed from 1) S186 S189 D397 T400 W528 K595 K598
Enzyme Commision number 6.1.1.5: isoleucine--tRNA ligase.
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation
GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1ffy, PDBe:1ffy, PDBj:1ffy
PDBsum1ffy
PubMed10446055
UniProtP41972|SYI1_STAAU Isoleucine--tRNA ligase (Gene Name=ileS)

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