Structure of PDB 1dpj Chain A Binding Site BS01

Receptor Information

>1dpj Chain A (length=329) Species: 4932 (Saccharomyces cerevisiae)

GGHDVPLTNYLNAQYYTDITLGTPPQNFKVILDTGSSNLWVPSNECGSLA
CFLHSKYDHEASSSYKANGTEFAIQYGTGSLEGYISQDTLSIGDLTIPKQ
DFAEATSEPGLTFAFGKFDGILGLGYDTISVDKVVPPFYNAIQQDLLDEK
RFAFYLGDTSKDTENGGEATFGGIDESKFKGDITWLPVRRKAYWEVKFEG
IGLGDEYAELESHGAAIDTGTSLITLPSGLAEMINAEIGAKKGWTGQYTL
DCNTRDNLPDLIFNFNGYNFTIGPYDYTLEVSGSCISAITPMDFPEPVGP
LAIVGDAFLRKYYSIYDLGNNAVGLAKAI

Ligand information

>1dpj Chain B (length=29) Species: 4932 (Saccharomyces cerevisiae)

TDQQKVSEIFQSSKEKLQGDAKVVSDAFK

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1dpj The aspartic proteinase from Saccharomyces cerevisiae folds its own inhibitor into a helix.
• Resolution: 1.8 Å
• Binding residue (original residue number in PDB): Y9 L10 Y75 T77 T111 F114 R186 A188 Y189 T218 L220 Q244 S279 S281 M289 F291 V295
• Binding residue (residue number reindexed from 1): Y10 L11 Y76 T78 T112 F115 R190 A192 Y193 T221 L223 Q247 S282 S284 M292 F294 V298

Enzymatic activity

• Catalytic site (original residue number in PDB): D32 S35 N37 W39 Y75 D215 T218
• Catalytic site (residue number reindexed from 1): D33 S36 N38 W40 Y76 D218 T221
• Enzyme Commision number: 3.4.23.25: saccharopepsin.

Gene Ontology

Molecular Function

• GO:0004190 aspartic-type endopeptidase activity

Biological Process

• GO:0006508 proteolysis

Cellular Component

• GO:0000324 fungal-type vacuole

External links

• PDB: RCSB:1dpj, PDBe:1dpj, PDBj:1dpj
• PDBsum: 1dpj
• PubMed: 10655612
• UniProt: P07267|CARP_YEAST Saccharopepsin (Gene Name=PEP4)