Structure of PDB 1bsu Chain A Binding Site BS01

Receptor Information
>1bsu Chain A (length=236) Species: 562 (Escherichia coli) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKTYNINELNE
IPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDS
EDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGR
Ligand information
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB1bsu Structural and energetic origins of indirect readout in site-specific DNA cleavage by a restriction endonuclease.
Resolution2.0 Å
Binding residue
(original residue number in PDB)		T37 N70 K92 T93 T94 Y95 T106 G109 T111 S112 K119 S183 T186 N188
Binding residue
(residue number reindexed from 1)		T36 N69 K91 T92 T93 Y94 T105 G108 T110 S111 K118 S175 T178 N180
Binding affinityPDBbind-CN: Kd=230nM
Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1bsu, PDBe:1bsu, PDBj:1bsu
PDBsum1bsu
PubMed10074946
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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