Structure of PDB 7jt3 Chain 9 Binding Site BS01

Receptor Information

>7jt3 Chain 9 (length=97) Species: 83333 (Escherichia coli K-12)

ISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIRASSLPEYYK
ERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAMIKELTT

Ligand information

>7jt3 Chain 3 (length=1539)

aauugaagaguuugaucauggcucagauugaacgcuggcggcaggccuaa
cacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacga
guggcggacgggugaguaaugucugggaaacugccugauggagggggaua
acuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagagg
gggaccuucgggccucuugccaucggaugugcccagaugggauuagcuag
uaggugggguaacggcucaccuaggcgacgaucccuagcuggucugagag
gaugaccagccacacuggaacugagacacgguccagacuccuacgggagg
cagcaguggggaauauugcacaaugggcgcaagccugaugcagccaugcc
gcguguaugaagaaggccuucggguuguaaaguacuuucagcggggagga
agggaguaaaguuaauaccuuugcucauugacguuacccgcagaagaagc
accggcuaacuccgugccagcagccgcgguaauacggagggugcaagcgu
uaaucggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucag
augugaaauccccgggcucaaccugggaacugcaucugauacuggcaagc
uugagucucguagagggggguagaauuccagguguagcggugaaaugcgu
agagaucuggaggaauaccgguggcgaaggcggcccccuggacgaagacu
gacgcucaggugcgaaagcguggggagcaaacaggauuagauacccuggu
aguccacgccguaaacgaugucgacuuggagguugugcccuugaggcgug
gcuuccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaa
gguuaaaacucaaaugaauugacgggggcccgcacaagcgguggagcaug
ugguuuaauucgaugcaacgcgaagaaccuuaccuggucuugacauccac
ggaaguuuucagagaugagaaugugccuucgggaaccgugagacaggugc
ugcauggcugucgucagcucguguugugaaauguuggguuaagucccgca
acgagcgcaacccuuauccuuuguugccagcgguccggccgggaacucaa
aggagacugccagugauaaacuggaggaagguggggaugacgucaaguca
ucauggcccuuacgaccagggcuacacacgugcuacaauggcgcauacaa
agagaagcgaccucgcgagagcaagcggaccucauaaagugcgucguagu
ccggauuggagucugcaacucgacuccaugaagucggaaucgcuaguaau
cguggaucagaaugccacggugaauacguucccgggccuuguacacaccg
cccgucacaccaugggaguggguugcaaaagaaguagguagcuuaaccuu
cgggagggcgcuuaccacuuugugauucaugacuggggcgaagucguaac
aagguaaccguaggggaaccugcgguuggaucaccuccu

.......<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<.....
<<<.<<<..<<<..<<.<...<<<.<<<<<......>>>>>..>>>.>..
>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<..<<...
<<<<<<......>>>>>.>....>>.>.....<<<....>>>....<<<<
<.<.<<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<..
<<<<<<<.........>>>>>>>>>>......>>>..<<<<<<<<....>
>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>.
..>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>>>
>>..<<<<.......<<<....>>>......>>>>..<<.<<<<<.....
.<.<<<<<<<<.......>>>>>>>>.>........>>>>>....>>.<<
<<<(((...<<<<<.....<<.)))>>.......>>>>>>>>>>..>>>>
>>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<
<<<<......<<<<<<.....>>>>>>....>>>>>>>..>>>>>>>>>>
...<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>..
....>>>>>>>.>..........<<....>>.>>>>>>>..>>>>.>>>>
...>>>...>>>>....<<<<<<...<<...<<<<.<.....>.>>>>..
.>>>>>>>>..........<<<<<<..<<<<<<<<<<<<.....>>>>>>
>>>>>>...<<..))>>.....>>>>>>.>>>.<<<......<<<<....
>>>>....>>>..)))).].<<<<.<<<<<<<.<<.<<<<<<..<<<<<<
<<<<......<<........>>..........<<<<<<<.......<<<<
<<..<<<<<<<....>>>>>>>...<......>..>>>>>.>..<<<.<<
...<<<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..
>>>>>>.....<<<<.<<<<<<<...<...<<<.....>>>.>....>>>
>>>>.....<<<<<.....>>>>>........>>>>.........>>>..
.>>>.>>>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<....
.<<<..<<...<<<....>>>...>>....>>>.....>>>>>>>.....
.<....<<<<<<<........>>>>>>>....>.....>>>>>>....<<
<<<<<.........>>>>>>>......>>...>>>>>>>>>>.>.....<
..<<.<.<.<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<...<<..
..>>..>>>>>>>>>...>>>>>>>>>>>>..>>>.>>.>..>.>>...>
.....<<<<<<<<<....>>>>>>>>>............

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7jt3 ArfB can displace mRNA to rescue stalled ribosomes
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): Q28 K32
• Binding residue (residue number reindexed from 1): Q25 K29

Enzymatic activity

• Enzyme Commision number: 3.1.1.29: peptidyl-tRNA hydrolase.

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0004045 aminoacyl-tRNA hydrolase activity
• GO:0016787 hydrolase activity
• GO:0043022 ribosome binding

Biological Process

• GO:0006415 translational termination
• GO:0006417 regulation of translation
• GO:0072344 rescue of stalled ribosome

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7jt3, PDBe:7jt3, PDBj:7jt3
• PDBsum: 7jt3
• PubMed: 33144582
• UniProt: P40711|ARFB_ECOLI Peptidyl-tRNA hydrolase ArfB (Gene Name=arfB)