Structure of PDB 5mlc Chain 9 Binding Site BS01

Receptor Information
>5mlc Chain 9 (length=107) Species: 3562 (Spinacia oleracea) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
SLIETNTKQRMEKTIETIRSNFNSVRLTSDRRKELAKVVSKLAEEGKVAV
RNIRRDALKSYEKLEKEKKLSEDNVKDLSADLQKLTDEYMKKVESIYKQK
EQELMKV
Ligand information
>5mlc Chain A (length=2750) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
uucaaacgaggaaaggcuuacgguggauaccuaggcacccagagacgagg
aagggcguauuaaucgacgaaaugcuucggggaguugaaaauaagcagag
auccggagauucccgaauaggucaaccuuucgaacuucugcugaauccau
gggcaggcaagagacaaccuggcgaacugaaacaucuuaguagccagagg
aaaagaaagcaaaagcgauucccguaguagcggcgagcgaaaugggagca
gccuaaaccgugaaaacgggguugugggagaggcugcuaggcgaaucagu
ggagugcggaacccuagauggugaaaguccaguagccgaaagcaucacuc
ugacccgaguagcauggggcacguggaaucccgugugaaucagcaaggac
caccuugcaaggcuaaauacuccugggugaccgauagcgaaguaguaccg
ugagggaagggugaaaagaacccccaucggggagugaaauagaacaugaa
accguaagcucucaagcagugggagggggaccagacccugaccgcgugcc
uguugaagaaugagccggcgacucauaggcaguggcuugguuaagggaac
ccaccggagccguagcgaaagcgagucuucauagggcaauugucacugcu
uauggacccgaaccugggugaucuauccaugaccaggaugaagcuugggu
gaaacuaaguggagguccgaaccgacugauguugaagaaucagcggauga
guugugguuaggggugaaaugccacucgaacccagagcuagcugguucuc
cccgaaaugcguugaggcgcagcaguugacuggacaucuagggguaaagc
acuguuucggugcgggccgcgagagcgguaccaaaucgaggcaaacucug
aauacuagauaugaccuaggggucaaggucggccagugagacgauggggg
auaagcuucaucgucgagagggaaacagcccggaucaccagcuaaggccc
cuaaaugaccgcucagugauaaaggagguaggggugcagagacagccagg
agguuugccuagaagcagccacccuugaaagagugcguaauagcucacug
aucgagcgcucuugcgccgaagaugaacggggcuaagcggucugccgaag
cugugggauguaaaaaaacaucgguaggggagcguuccguguuagggaga
aacgcgugcgugagccgcguuggacgaagcggaagcgagaaugucggcuu
gaguaacgcaaacauuggugagaauccaaugccccgaaaaccuaaggguu
ccuccgcaagguucguccacggagggugagucagggccuaagaucaggcc
gaaaggcguagucgauggacaacaggugaauauuccuguacuaccccuug
uuggucccgagggacggaggaggcuagguuagccgaaagaugguuaucgg
uucaaggacgcaaggugaagggguagagaaaaugccucgagccaauguuc
gaguaccaggcgcuacggcgcugaaguaaccgaugccauacucccaggaa
aagcucgaacgaccuucaacaaaaggguaccuguacccgaaaccgacaca
gguagguagguagagaauaccuaggggcgcgagacaacucucucuaagga
acucggcaaaauagccccguaacuucgggagaaggggugcccccucacaa
agggggucgaagugaccaggcccgggcgacuguuuaccaaaaacacaggu
cuccgcaaagucguaagaccauguaugggggcugacgccugcccagugcc
ggaaggucaaggaaguuggugaccugaugacaggggagccggcgaccgaa
gccccggugaacggcggccguacgguccuaagguagcgaaauuccuuguc
ggguaaguuccgacccgcacgaaaggcguaacgaucugggcacugucucg
gagagaggcucggugaaauagacaugucugugaagaugcggacuaccugc
accuggacagaaagacccuaugaagcuuuacuguucccugggauuggcuu
ugggcuuuuccugcgcagcuuagguggaaggcgaagaaggcccccuuccg
ggggggcccgagccaucagugagauaccacucuggaagagcuagaauucu
aaccuugugucaggaccuacgggccaagggacauucucagguagacaguu
ucuauggggcguaggccucccaaaagguaacggaggcgugcaaagguuuc
cucgggccggacggagauuggcccucgagugcaaaggcagaagggagcuu
gacugcaagacccacccgucgagcagggacgaaagucggccuuagugauc
cgacggugccgaguggaagggccgucgcucaacggauaaaaguuacucua
gggauaacaggcugaucuuccccaagaguucacaucgacgggaagguuug
gcaccucgaugucggcucuucgccaccuggggcuguaguauguuccaagg
guugggcuguucgcccauuaaagcgguacgugagcuggguucagaacguc
gugagacaguucgguccauauccggugugggcguuagagcauugagagga
ccuuucccuaguacgagaggaccgggaaggacgcaccucugguguaccag
uuaucgugcccacgguaaacgcuggguagccaagugcggagcggauaacu
gcugaaagcaucuaaguaguaagcccaccccaagaugagugcucuccuau
..............<<<<<<<<<<.....<<..<<<<<<<<<<<.<....
..<<<<<<.....((.>>>.....<<<<<<<..<<.))......>>....
.>>>>>>>...>>>....<<<....>>>......<<.<<<<<........
.>>>>>...>>.>....<<<<<<.....<<....>>......>>>>>.>.
........<<....>>..<<<<<<.....<<.....>>....>>>>>>..
<<<....<<<......>>>......<<<.<<...<<<<.<<<...<<.<<
.....>>.>>((......<<<.....))>>>...>>>...>>>>....>>
...>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>........<<<<..<<<<.......>>>>..>>>>...<.
<........>.>.<<<<<<....<<<<<<<<<<<<...<<<...<<....
...>>...>>>...<<....>>..<<<<.....>>>>.....>>>>>>>>
>>>>..<<.<...<<<<<<.....<.<<<<<<<..<<<<...<<<<<<..
....>>>>>>...>.>>>...<<<.<<<<<........>>>>>>>>....
>>>>>>>.>...<<<.....>>>......>>>>>>......>.>>..<<<
<<<....<<<<<....>>>>><<.<<<<<<<....<<<<<<.....<<<.
..<<<<<<<<<..<<<<<<<....>>>>>.>>..>>>>>>>>>..>>.>.
....>>>>>>..<<<<....>>>>..>>>>>>>..>>..<<<<<<<<<<.
.....>>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<((
(.....<<<<<<...........<<..<<<<<<.<<<.......<.<<<.
<.....<<.........>>.....>.........<<......>>...>>>
.>...>>>.>>>>>>.>>..........))).....>>>>>>.>>>...>
>>>>>><<<<........>>>>....>>>>>><.<<<<<<..........
.<..<..<<....>>.>...>.......>>>>>>..>.....>>>>>><.
.....<<.....<<<<<<.......>>>>>><<<<....<<<<<.<<...
<<<<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<
....>>>...>>>...>>...<<<<<........>>>>>))<<<<<.<<<
<<<<<<.<<<<....<<<<..<<<..<<<<<<<<<<.....(((..>>>>
><...<<<<<...<<<....<<<<<.......>>>>>...>>>..>>>>>
....><..<<<<<....>>>>>...>>>>>>...>>>...>>>>......
...>>>>...>>>>...>>>>>..>>>>>>>>>..>>.....<<......
>>>>>>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<..
.............<<<<<.....<..>......>>>>><<<<<<<.....
>>>>>>>............<<<<<<<<<..<<<.........>>>..<<<
<<<<.....<<<....>>>.......>>>>>>>.<<<<<.<<<..<<<<<
<<..<<<...<<..<<<<<<..<<<<....>>>>...>>>>>>..>>...
>>>>>>>>..>>>>><<<<<..>>>>>........<...........<<<
<<<.....>>>>>>.....>....>>>>>..>>.>>>>>>>.....>>.>
>>>>>>>.>>>>>>.....>.....<<<<<........>>>>>..<<<<<
<<<.<<<..<......<<<<.<<<<<<..<<<<<..<<<<<<<..<<.<<
<.<<<<<<<<<...........<<<<...<<<......<<<<<<<<....
>>>>>>>>...>>>.............>>>>..>>>>>>>>>.>>>.>>.
..<<<<<.<<<..........>>>>>>>>.....>>>>>>>...>>>>>.
.<<<<.....>>>><<<<<<...........>>>>>>..<<...<<<<<<
<..<<<<<<<.......>>>>>>>....<<...(((>>....>>>>>>>.
..<<<<..<<<......>>>..>>>>.<<<....>>>.>>))).......
<<<<<<<.<<..>>>>..>>>>>>>>>.............>>>>..>>>>
>>.......<<<<.<.<<<<<<...<<.......>>...>>>>>>...>>
>>.>.......<<<<<.<.<<<<..<<<<<<<<........>>>>>..>>
>.<<<<<.....>>>>>.....>>>>...>...>>>.>>....<<<<<<<
....>>>.>>>>....>...>>>>>>>>>>>....<<<<<<<...<.<<.
.<<<<<<<...............>>>>>>>.<<<<<...<<<<...<<<<
<<<<<<<))).>>>>>>...>>>>>...>>>>.>>>>>..<.<<...<<<
<<<...........>>>>>>...>>>.>>.>.......>>>>>>>.....
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5mlc Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.
Resolution3.6 Å
Binding residue
(original residue number in PDB)		E208 V212 R215 R218 R219 K223 S235 E236 D237
Binding residue
(residue number reindexed from 1)		E44 V48 R51 R54 R55 K59 S71 E72 D73
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0043023 ribosomal large subunit binding
Biological Process
GO:0006412 translation
GO:0032544 plastid translation
Cellular Component
GO:0009507 chloroplast
GO:0009570 chloroplast stroma

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:5mlc, PDBe:5mlc, PDBj:5mlc
PDBsum5mlc
PubMed27986857
UniProtP82231|RRFC_SPIOL Ribosome-recycling factor, chloroplastic (Gene Name=RRF)

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