Structure of PDB 3j78 Chain 81 Binding Site BS01

Receptor Information

>3j78 Chain 81 (length=109) Species: 4932 (Saccharomyces cerevisiae)

LKDVVTREYTINLHKRLHGVSFKKRAPRAVKEIKKFAKLHMGTDDVRLAP
ELNQAIWKRGVKGVEYRLRLRISRKRNEEEDAKNPLFSYVEPVLVASAKG
LQTVVVEED

Ligand information

>3j78 Chain 2S (length=3302)

uugaccucaaaucagguaggaguacccgcugaacuuaagcauaucaauaa
gcggaggaaaagaaaccaaccgggauugccuuaguaacggcgagugaagc
ggcaaaagcucaaauuugaaaucugguaccuucggugcccgaguuguaau
uuggagagggcaacuuuggggccguuccuugucuauguuccuuggaacag
gacgucauagagggugagaaucccguguggcgaggagugcgguucuuugu
aaagugccuucgaagagucgaguuguuugggaaugcagcucuaagugggu
gguaaauuccaucuaaagcuaaauauuggcgagagaccgauagcgaacaa
guacagugauggaaagaugaaaagaacuuugaaaagagagugaaaaagua
cgugaaauuguugaaagggaagggcauuugaucagacaugguguuuugua
uuucacuggccagcaucaguuuugguggcaggauaaauccauaggaaugu
agcuugccucgguaaguauuauagccugugggaauacugccagcugggac
ugaggacugcgacguaagucaaggaugcuggcauaaugguuauaugccgc
ccgucuugaaacacggaccaaggagucuaacgucuaugcgaguguuuggg
uguaaaacccauacgcguaaugaaagugaacguagguuggggccucgcaa
gaggugcacaaucgaccgauccugaugucuucggauggauuugaguaaga
gcauagcuguugggacccgaaagauggugaacuaugccugaauaggguga
agccagaggaaacucugguggaggcucguagcgguucugacgugcaaauc
gaucgucgaauuuggguauaggggcgaaagacuaaucgaaccaucuagua
gcugguuccugccgaaguuucccucaggauagcagaagcucguaucaguu
uuaugagguaaagcgaaugauuagagguuccggggucgaaaugaccuuga
ccuauucucaaacuuuaaauauguaagaaguccuuguuacuuaauugaac
guggacauuugaaugaagagcuuuuagugggccauuuuugguaagcagaa
cuggcgaugcgggaugaaccgaacguagaguuaaggugccggaauacacg
cucaucagacaccacaaaagguguuaguucaucuagacagccggacggug
gccauggaagucggaauccgcuaaggaguguguaacaacucaccggccga
augaacuagcccugaaaauggauggcgcucaagcguguuaccuauacucu
accgucaggguugauaugaugcccugacgaguaggcaggcguggagguca
gugacgaagccuagaccguaaggucgggucgaacggccucuagugcagau
cuuggugguaguagcaaauauucaaaugagaacuuugaagacugaagugg
ggaaagguuccacgucaacagcaguuggacguggguuagucgauccuaag
agauggggaagcuccguuucaaaggccugauuuuaugcaggccaccaucg
aaagggaauccgguuaagauuccggaaccuggauauggauucuucacggu
aacguaacugaauguggagacgucggcgcgagcccugggaggaguuaucu
uuucuucuuaacagcuuaucaccccggaauugguuuauccggagaugggg
ucuuauggcuggaagaggccagcaccuuugcuggcuccggugcgcuugug
acggcccgugaaaauccacaggaaggaauaguuuucaugccaggucguac
ugauaaccgcagcaggucuccaaggugaacagccucuaguugauagaaua
auguagauaagggaagucggcaaaauagauccguaacuucgggauaagga
uuggcucuaagggucggguagugagggccuuggucagacgcagcgggccu
uguuguagacggccuugguaggucucuuguagaccgucgcuugcuacaac
gaucaacuuagaacugguacggacaaggggaaucugacugucuaauuaaa
acauagcauugcgauggucagaaagugauguugacgcaaugugauuucug
cccagugcucugaaugucaaagugaagaaauucaaccaagcgcggguaaa
cggcgggaguaacuaugacucucuuaagguagccaaaugccucgucaucu
aauuagugacgcgcaugaauggauuaacgagauucccacugucccuaucu
acuaucuagcgaaaccacagccaagggaacgggcuuggcagaaucagcgg
ggaaagaagacccuguugagcuugacucuaguuugacauugugaagagac
auagaggguguagaauaagugggagcuucggcgccagugaaauaccacua
ccuuuauaguuucuuuacuuauucaaugaagcggagcuggaauucauuuu
ccacguucuagcauucaaggucccauucggggcugauccggguugaagac
auugucagguggggaguuuggcuggggcggcacaucuguuaaacgauaac
gcagauguccuaaggggggcucauggagaacagaaaucuccaguagaaca
aaaggguaaaagcccccuugauuuugauuuucagugugaauacaaaccau
gaaaguguggccuaucgauccuuuagucccucggaauuugaggcuagagg
ugccagaaaaguuaccacagggauaacuggcuuguggcagucaagcguuc
auagcgacauugcuuuuugauucuucgaugucggcucuuccuaucauacc
gaagcagaauucgguaagcguuggauuguucacccacuaauagggaacgu
gagcuggguuuagaccgucgugagacagguuaguuuuacccuacugauga
auguuaccgcaauaguaauugaacuuaguacgagaggaacaguucauucg
gauaauugguuuuugcggcugucugaucaggcauugccgcgaagcuacca
uccgcuggauuauggcugaacgccucuaagucagaauccaugcuagaacg
cggugauuucuuugcuccacacaauauagauggauacgaauaaggcgucc
uuguggcgucgcugaaccauagcaggcuagcaacggugcacuuggcggaa
aggccuugggugcuugcuggcgaauugcaaugucauuuugcguggggaua
aaucauuuguauacgacuuagauguacaacgggguauuguaagcaguaga
guagccuuguuguuacgaucugcugagauuaagccuuuguugucugauuu
gu

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>>.(.......<<<......)>>>....>>>>>>>>..>>>>>>>>....
>>>>>>>>>>>........<<<<<<<........>>>>>>>.....((((
(.<<<<<<<)))))..]]]...............................
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.<<<<<......>>>>>.......>>>>>>>.>.>.>>>>>.>>>>>>>>
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<<<<........>>>><<<<<<....<<<<<<.<<<<<<..<<<...<<<
<.....>>>>...>>>...<<....>>.......<<<<<..<<<<<....
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>>>>>>.>>>>>>..<<.<..<<<<<<<.....<<<<<<<<<..<<<...
.<<<<<<<....>>>>>>>.....>>>...<<<..<<.<<........>>
>>.>>>....>>>>>>>>>...<<.......>>......>>>>>>...>.
.>.>>..<<<<<<....<.<<<....>>>.><<<<<<<<<<...<<<.<<
<<<<<.....<<<....<<<....<<<<...<<<<<.......>>>>>.>
>>>....>>>..>.>>.....>>>>>...<<<<.<<<<.<......>>>>
>.>>>>.....>>>>>.>>>>>>>>.>>..<<<<.<<<<<.....>>>>>
.>>>>....<<<......>>>...<<<<<<<..<<<<(((.....<<<<<
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..>>>.......<<<<<<...<<<<..<<<<<<.<<<<<<<<<<..{{{.
.>>>>>>....<<<<<....<<<<<<....<<<.....>>>....>>>>>
>.....>>>>>.....<<<<<<<......>>>>>>>>>>>...>>>>>>.
.>>>>.........>>>>>>..........>.>..>>>>>>>>>>>>..>
>.....<<......>>>>>>>.<<<<.....>>>>..>>>>..<<<<...
..<<<<<<<.<<...............<<<<<.....<..>......>>>
>><<.<<<<<<<<<<.....<<<<.<<<<....<...<<<<<<......>
>>>.>>..>.>>.>>.....................>>>.>.........
>>>>..>>>>>>.>>............<<<<<<<<<..<<<.........
>>>..<<<<<<<....<<<<.....>>>>......>>>>>>>.<.<<..<
<<..<<<<<<.<...<<.....<<<<....>>>>.....>>.>>>>>..>
>>>><<<<<<.......>>>>>>........<...........<<<<<..
.....>>>>>.....>.....>>.>..>>.>>>>>>>.....>>.>>>>>
>>..>>>>>>...>>>....<<<<<........>>>>>.....[[[[..{
{{.{{......[[[[.{{[[[[..<<<<<.<<<<<<<<..<<<<<<<<<<
.<.<..<<...........<..........................>...
>>..>>..>>>>>>>>>>...<<<<<.....<<<<..<<<<<......>>
>>>...............<<<<<<....>>>>>>..>>>>..>>>>>...
..>>>>>>>>..>>>>>...<<<.....>>>..<<<<<<...........
>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.
..(((>>....>>>>>>>...<<<.<<<<<.......>>>>.>>>>.<<<
....>>>.>>))).......((((((..(((..>>>>>>>))))))))).
.........]]]]..}}]]]].......<<.<...<<<<<<...<<....
...>>...>>>>>>....>..>>.......<<<<<..<<<<<..<.<<<<
<<<......>>>>>>..>.>.<<<.<.....>.>>>.....>>>>>....
..>>>.>>....<<<<<<<....>>>.>>>>....}}..}}}.]]]]...
..<<<<<<<<..<<<<...<<<<<<...............>>>>>>..<<
<<<...<<<<....<<<<<<<<<.}}}..>>>>..>>>>>...>>>>..>
>>>>.<<<<...<<<<<...........>>>>>...>>>>.>>>>....>
>>>>>>>.......<<<<<<.<<<.<<....<<..........<<<<<<.
....>>>>>>......>>.......<<<<<<...<<<<<.<..<<<....
..>>>..>.>>>>>.>>>>>>.............>>.>>>.>>>>>>..<
<<<<.<.............>.....<<<<<<<<<.<<<....<<<<....
<<<<<......>>>>>...>>>>...>>>....>>>>.>>>>>..>>>>>
..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 3j78 Structures of Yeast 80S Ribosome-tRNA Complexes in the Rotated and Nonrotated Conformations.
• Resolution: 6.3 Å
• Binding residue (original residue number in PDB): R10 T13 H17 K18 R19 H21 G22 V23 S24 F25 K26 V33 K34 L51 N56 W60 R62 Y69 R70 G103 L104 Q105 T106 V107 V108
• Binding residue (residue number reindexed from 1): R7 T10 H14 K15 R16 H18 G19 V20 S21 F22 K23 V30 K31 L48 N53 W57 R59 Y66 R67 G100 L101 Q102 T103 V104 V105

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0006450 regulation of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:3j78, PDBe:3j78, PDBj:3j78
• PDBsum: 3j78
• PubMed: 25043550
• UniProt: P0C2H8|RL31A_YEAST Large ribosomal subunit protein eL31A (Gene Name=RPL31A)