Structure of PDB 8a63 Chain 8 Binding Site BS01

Receptor Information

>8a63 Chain 8 (length=63) Species: 169963 (Listeria monocytogenes EGD-e)

PKMKTHRGSAKRFKRTGSGKLKRRHGFTSHMFANKSQKQKRKLRKSAMVS
AGDFKRIRQMVAK

Ligand information

>8a63 Chain A (length=2908)

uuaaguuagaaagggcgcacgguggaugccuuggcacuaggagccgaaga
aggacgggacuaacaccgauaugcuuuggggagcuguacguaagcguuga
uccagagauuuccgaaugggggaacccacuaucuuuagucggauaguauc
cuuacgugaauacauagcgugaggaaggcagacccagggaacugaaacau
cuaaguaccuggaggaagagaaagaaaaaucgauuuccugaguagcggcg
agcgaaacggaaagagcccaaaccaagaagcuugcuucuugggguuguag
gacacucuauauggaguuacaaaagaaaguuauaaaugaagcggucugga
aaggcccgccaaagacgguaacagcccgguaguugaaauggcuuucccuc
cagaguggauccugaguacggcggaacacgugaaauuccgucggaauccg
ggaggaccaucucccaaggcuaaauacucccuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaacagu
uccugaaaccgugugccuacaaguaguuagagcccguuaaugggugauag
cgugccuuuuguagaaugaaccggcgaguuacgauuuguugcaagguuaa
gcggaaaaagcggagccguagcgaaagcgagucugaauagggcgcauaag
uaacaggucguagacccgaaaccaggugaucuacccauguccaggaugaa
gguaagguaauacuuacuggagguccgaacccacgcacguugaaaagugc
ggggaugagguguggguagcggagaaauuccaaucgaacuuggagauagc
ugguucucuccgaaauagcuuuagggcuagccucgagguaaagagucaug
gagguagagcacuguuuggacuaggggcccuucucggguuaccgaauuca
gauaaacuccgaaugccauguacuuauacucgggagucagacugcgagug
auaagauccguagucgaaagggaaacagcccagaccaccaguuaaggucc
ccaaauauauguuaaguggaaaaggaugugggguugcuuagacaaccagg
auguuggcuuagaagcagccaccauugaaagagugcguaauagcucacug
gucgagugaccccgcgccgaaaauguaccggggcuaaacauauuaccgaa
acuguggaugaaccgguucgugguaggagagcguucuaagggcggugaag
ucagaccggaaggacugguggagcgcuuagaagugagaaugccgguauga
guagcgaaagaagggugagaaucccuuccaccgaauaucuaagguuuccu
gaggaaggcucguccgcucaggguuagucgggaccuaagccgaggccgau
aggcguaggcgauggacaacagguagagauuccuguaccagugcuaauug
uuuaaccgauggggugacacagaaggauagggaaucgcacgaauggaaau
gugcguccaagcagugagugugagaaguaggcaaauccgcuucucgcgaa
gcaugagcugugauggggaacggaaguuccugauuucacgcugucaagaa
aagccucuaggaagaguaguacugcccguaccgcaaaccgacacagguag
augaggagagaauccuaaggugagcgagagaacucucguuaaggaacucg
gcaaaaugaccccguaacuucgggagaaggggugcucuauuagggugcaa
gcccgagagagccgcagugaauaggcccaggcgacuguuuagcaaaaaca
caggucucugcaaaaccguaaggugacguauaggggcugacgccugcccg
gugcuggaagguuaagaggagugcuuagcuucggcgaagguacgaauuga
agccccaguaaacggcggccguaacuauaacgguccuaagguagcgaaau
uccuugucggguaaguuccgacccgcacgaaaggcgcaacgaucugggca
cugucucaacgagagacucggugaaauuauaguaccugugaagaugcagg
uuacccgcgacaggacggaaagaccccguggagcuuuacugcaaccugau
auggaauguuuguaccgcuuguacaggauagguaggagccgaagagacgu
gugcgcuagcauacgaggaggcaauggugggauacuacccuggcuguaug
accauucuaacccgccacgcuuagcgcguggggagacagugucagguggg
caguuugacuggggcggucgccuccuaaagaguaacggaggcgcccaaag
guucccucagaauggauggaaaucauucgcagaguguaaaggcacaaggg
agcuugacugcgagacugacaagucgagcagggacgaaagucgggcuuag
ugauccggugguuccgcauggaagggccaucgcucaacggauaaaagcua
ccccggggauaacaggcuuaucucccccaagaguccacaucgacggggag
guuuggcaccucgaugucggcucgucgcauccuggggcuguagucggucc
caaggguugggcuguucgcccauuaaagcggcacgcgagcuggguucaga
acgucgugagacaguucggucccuauccgucgcgggcgcaggaaauuuga
gaggagcuguccuuaguacgagaggaccgggauggacacaccgcuggugu
accaguuguuccgccaggagcaucgcuggguagcuauguguggcagggau
aaacgcugaaagcaucuaagcgugaagccccccucaagaugagauuuccc
auuucuucggaaaguaagaucccugaaagaugaucagguagauagguuug
gaguggaaguguagcgauacauggagcggacaaauacuaaucgaucgagg
acuuaacc

<<<<<<......<<<<<<<<<<<.....[[..[[<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>>>.>>....<<<<<<.....<<....>
>......>>>>>.>.........<<....>>..<<<<<......<<....
.>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<<
<<<<<<<<....<<<<........<<<<<<<<<.<<<.....<<<<....
..>>>>.((......<<<.....))>>>...>>>...>>>>>>>>>.>>>
>>>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.]]...........
....<<....>>....]].........<<<<....>>>>...........
.......>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<<<<<<<..<<<...
<<.......>>...>>>...<<....>>..<<<<.....>>>>......>
>>>>>>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....
<<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>
>>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>
.>>..<<<<<<....<<<<<....>>>>><<<<<<<<.....<<<<<<<<
<.....<<<...<<<<<<<<.<.<<<<<.<......>>>>.>>.>.>>>>
>>>>..>>.>.....>>>>>.>>>>...>>>>>>.>>..<<<<<<<.<<.
.....>>.>>>>>>>....<<<......>>>...<<<<.<<<...<<<((
(.....<<<<<<...........<<..<<<<<<<<<<.......<<<<..
<<...<<<<.......>>>>...>>.........<<......>>....>>
>>...>>>>>>>>>>.>>...........))).....>>>>>>.>>>...
>>>>>>><<<<<<..>>>>>>....>>>>>>..<<<<<<<.<<......<
<<<<.<<....>>.>>>>>...>>.>>>>>>>........>>>>>><...
...<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<
<<<...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<...
.>>>...>>>...>>...<<<<<........>>>>>))<<<<<<<<.<<.
.....<<...<<<<<...<<<<..<<<..<<<<<.<<<<<...(((....
>>>>>.<<..<<<<<..<<<<<<<<<<<.<<.....>>>>>>>>>.....
>>>>..>>>>>...>>..........>>>>>...>>>...>>>>......
..>>>>>..>>..>>.>>>>>>>>>>>>..>>.....<<......>>>>>
>>.<<<<.....>>>>..>>>>..<<<<....<<<<<<<<.<<.......
..<.....<<<<<...............>>>>><<<<<....<<<<....
>>>>...>>>>>............<<<<<<<<<..<<<.........>>>
..<<<<<<<.....<<<....>>>.......>>>>>>>..<<<<.<<<..
<<<<<<<..<<<<..<<...<<<<<..<<....>>...>>>>>...>>..
>>>>>>>>>..>>>>><<<<<.........>>>>>........<......
.....<<<<<<.....>>>>>>.....>....>>>>...>>.>>>>>>>.
.>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<........>>>>
>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<<<<<<<
<<.<<<<<.<..<<<.<<............<<<....<<........<<<
<.<<....>>.>>>>.....>>..............>>>...>>.>>>..
>.>>>>>....<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>
>>>>..<<<<.....>>>><<<<<<...........>>>>>>..<<...<
<<<<<<...<<<<<<.......>>>>>>.....<<.[.(((>>....>>>
>>>>...<<<<..<<<<....>>>>..>>>>.<<<....>>>.>>)))].
.....<<<<<<<.((..>>>>..))>>>>>>>.............>>>>.
.]]>>>>.......<<<<...<<<<<<...<<.......>>...>>>>>>
....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<......>>>>>
>>..>>.<<<<<.....>>>>>.....>>>>>..>...>>>.>>....<<
<<<<<....>>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...
<<<<..<<<<<<<...............>>>>>>>.<<<<<...<<<<..
.<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<..
..<<<<<...........>>>>>....>>>.>>>>.......>>>>>>>.
.<<<<....>>>>....<<<<<<<<<.......>>>>>....<<<<<<<<
..<<....<<<<<....>>>>>...>>...>>>>>.>>>...>>>>....
>>>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8a63 Structural basis for HflXr-mediated antibiotic resistance in Listeria monocytogenes.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): P2 K3 M4 K5 T6 H7 R8 K12 R13 K15 T17 G18 S19 K23 R24 H26 G27 F28 S30 H31 M32 F33 N35 Q38 K39 Q40 K41 R42 K43 R45 K46 S51 G53 D54 K56 R57 R59 Q60 K64
• Binding residue (residue number reindexed from 1): P1 K2 M3 K4 T5 H6 R7 K11 R12 K14 T16 G17 S18 K22 R23 H25 G26 F27 S29 H30 M31 F32 N34 Q37 K38 Q39 K40 R41 K42 R44 K45 S50 G52 D53 K55 R56 R58 Q59 K63

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8a63, PDBe:8a63, PDBj:8a63
• PDBsum: 8a63
• PubMed: 36300626
• UniProt: P0A491|RL35_LISMO Large ribosomal subunit protein bL35 (Gene Name=rpmI)