Structure of PDB 8wi7 Chain 7 Binding Site BS01

Receptor Information

>8wi7 Chain 7 (length=46) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

AKGKRTFQPNNRRRARVHGFRLRMRTRAGRAIVANRRSKGRRALTA

Ligand information

>8wi7 Chain A (length=3025)

aaguguuuaagggcgcaugguggaugccuuggcacugggagccgaugaag
gacguaggaggcugcgauaagccucggggagcugucaaccgagcguugau
ccgaggauguccgaauggggaaacccggcacgagugaugucgugucacca
ggcgcugaauauauaggcgucuggggggaacgcggggaagugaaacaucu
caguacccguaggaagagaaaacaaaaugugauuccgugaguaguggcga
gcgaaagcggaggauggcuaaaccguaugcaugugauaccggguaggggu
ugugugugcgggguugugggaccuaucuuuccggcucuaccuggcuggag
ggcagugagaaaauguugugguuagcggaaauggcuugggauggccugcc
guagacggugagagcccgguacgugaaaacccgacgucugucuugauggu
guucccgaguagcagcgggcccguggaaucugcugugaaucugccgggac
cacccgguaagccugaauacuucccagugaccgauagcggauuaguaccg
ugagggaauggugaaaaguaccccgggaggggagugaaagaguaccugaa
accgugcgcuuacaauccgucagagcccucgacgugucguggggugaugg
cgugccuuuugaagaaugagccugcgagucagggacaugucgcgagguua
acccgggugggguagccgcagcgaaagcgagucugaauagggcguaucca
cacaagagugugugguguagugguguguucuggacccgaagcggagugau
cuacccauggccagggugaagcgcggguaagaccgcguggaggcccgaac
ccacuuagguugaagacugaggggaugagcuguggguaggggugaaaggc
caaucaaacuccgugauagcugguucuccccgaaaugcauuuaggugcag
cgucgcauguuucuugccggagguagagcuacuggauggccgaugggccc
cacaggguuacugacgucagccaaacuccgaaugccgguaaguccaagag
ugcggcagugagacggcgggggauaagcuccgugcgucgagagggaaaca
gcccagaucgccggcuaaggccccuaagcgugugcuaaguggaaaaggau
gugcagucgcgaagacaacacuggucaagugauugugcgccgauaaugua
gcggggcucaagcacaccgccgaagccgcggcagccaacguguuggcugg
guaggggagcguccugcauccggugaagccgccgagugaucgaguggugg
agggugugggagugagaaugcaggcaugaguagcgauuaggcaagugaga
accuugcccgccgaaagaccaaggguuccugggccaggccaguccgccca
gggugagucgggaccuaaggcgaggccgacaggcguagucgauggacaac
ggguugauauucccguacccguguaugugcguccaugaugaaucagcggu
acuaaccauccaugcaugggaccuucguugguaguagucaagcgaugggg
ugacgcaggaagguagccguaccggucagugguaauaccgggguaagccu
guagggagucagauagguaaauccgucuggcauauauccugagaggugau
gcauagccgagugaggcgaauucggugauccuaugcugccgagaaaagcc
ucuagcgaggacauacacggcccguaccccaaaccaacacagguggucag
guagagaauacuaaggcguacgagugaacuaugguuaaggaacucggcaa
aaugcccccguaacuucgggagaagggggacccacauggcguguaagccu
uuacggcccaagcgugaguggguggcacaaaccagugagaagcgacuguu
uacuaaaaacacagguccgugcgaagucgcaagacgauguauacggacug
acgccugcccggugcuggaagguuaagaggacccguuaacucccuuuggg
ggugaagcggagaauuuaagccccaguaaacggcggugguaacuauaacc
auccuaagguagcgaaauuccuugucggguaaguuccgaccugcacgaau
ggcguaacgacuucucaacugucucaaccauagacucggcgaaauugcac
uacgaguaaagaugcucguuacgcgcggcaggacgaaaagaccccgggac
cuucacuacaacuugguauuggugcucgauacgguuuguguaggauaggu
gggagacugugaagcucacacgccaguguggguggagucguuguugaaau
accacucugaucguauugggccucuaaccucggaccguauauccgguuca
gggacagugccugguggguaguuuaacuggggcgguugccuccuaaaaug
uaacggaggcgcccaaagguucccucaaccuggacggcaaucagguguug
aguguaagugcacaagggagcuugacugcgagacggacaugucgagcagg
gacgaaagucgggacuagugauccggcaccucugaguggaaggggugucg
cucaacggauaaaagguaccccggggauaacaggcugaucuuccccaaga
guccauaucgacgggaugguuuggcaccucgaugucggcucgucgcaucc
uggggcuggagcaggucccaaggguugggcuguucgcccauuaaagcggc
acgcgagcuggguuuagaacgucgugagacaguucggucucuauccgccg
cgcgcgucagaagcuugaggaaaccugucccuaguacgagaggaccggga
cggacgaaccucugguauaccaguugucccaccaggggcacggcuggaua
gccacguucggacaggauaaccgcugaaagcaucuaagcgggaaaccucu
uccaagaccaggcuucucacccucuaggagggauaaggccccccgcagac
cacgggauugauagaccagaccuggaagccuaguaauaggugcagggaac
uggcacuaaccggccgaaaacuuac

<<<<......<<<<<<<<<<<.....<<..<<<<<<<<<<<<<......<
<<<<<.[[.(((.>>>.....<<<<<<<..<<<)))..]].>>>.....>
>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<<
<<<<<<.........>>>>>>>>>.>>...<<<<<<.....<<....>>.
.....>>>>>.>.........<<.....>>..<<<<<<.....<<.....
>>....>>>>>>...<<<....<<<<<<<<<<.........(((((....
.>>>>>>>>>>......<<<<..<.....<<<<<<<))))).>>>>>>><
<<<<<.......<<<<<<.<<<<.<<<.....<<<<......>>>>.((.
.....<<<.....))>>>...>>>...>>>>>>>>>>>>>>>>.....>.
..>>>>.....<<<<<<<<.........>>>>>>>>......<<<<<..<
<<<>>>>>..>>>......>>.>>>>>>>.>>...............<<.
...>>....>>.........<<<<....>>>>..................
>>>>>>>>>>>.......<<<<..<<<<<<<<<..>>>>.>>>>>..>>>
>...<.<........>.>.<<<<<<....<<<.<<<<<<<<<<..<<<..
.<<<....>>>...>>>...<<....>>..<<<<.....>>>>....<<<
<<<<....>>>>>>>....>>>>>>>>>>>>>..<<.<..<<<<<<<...
..<<<<<<<<<..<<<....<<<<<<......>>>>>>.....>>>...<
<<.<<<<<........>>>>>>>>....>>>>>>>>>...<<<.....>>
>......>>>>>>...>..>.>>..<<<<<<....<<<<<....>>>>><
<<<<<<<<....<<<<<<<<.....<<<....<<<.<.<<......<<<<
....>>>>.......>>.>.>>>..>>.>.....>>>>>>>>.......>
>>>>>>.>>..<<<<<<<<<<......>>>>>>.>>>>....<<<.....
.>>>...<<<<.<<<...<<<(((.....<<<<<<...........<<..
<<<<<<<<<........<......>....>>>>>>>>>.>>.........
..)))......>>>>>>.>>>...>>>>>>><<<<<<<<..>>>>>>>>.
...>>>>>><.<<<<<<<<<<......<<<<<<<<....>>>.>>>>>..
.>>>>>>>>>>..>.....>>>>>><......<<.....<<<<<<.....
..>>>>>><<<<....<<<<<.<<...<<<<<<...<<.....>>.>>>>
>>....((<<<<.<<...<<<...<<<....>>>...>>>...>>...<<
<<<........>>>>>))<<<<<<<<<<.......<<<<..<<<<<<<<.
.....<<<<......>>>>.....>>>>>>>>....>>>>.<<...<<<<
<...<<<<..<<<..<<<<..<<<<<...((((...>>>>>.<<..<<<<
...<<<<<<<<<<<.<<.....>>>>>>>>>.....>>>>...>>>>...
>>...<<<......>>>.....>>>>...>>>...>>>>........>>>
>>..>>....>>>>>>>>>>>>>>..>>.....<<......>>>>>>>.<
<<<.....>>>>..>>>>..<<<<<...<<<<<<<<.<<.........<.
....<<<<<....<<..>>.....>>>>><<<<<<...<<<<....<<<.
....>>>....>>>>..>>>>>>............<<<<<<<<<..<<<.
........>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.
<<<<<.<<<..<<<<<<<..<<<..<<<....<<<<<.<<<<<<...>>>
>>>..>>>>>....>>>..>>>>>>>>..>>>>><<<<<<.......>>>
>>>........<...........<<<<<<.....>>>>>>.....>....
>>>>>..>>.>>>>>>>..>..>>.>>>>>>>>>>>>>>>.....>.((.
.<<<<<......))>>>>>..<<<<<<<<.<<<.<<......<<<<<<<<
<<..<<<<<.<<<.<<<<<.<<.<<<<<<<<<<<<..............<
<<..<<<......<<<<<<<<....>>>>>>>>...>>>...........
.>>>.....>>>>>>>>>>>>.>>...<<<.<<<<<<......>>>>>>>
>>....>>>>>.>>>..>>>>>..<<<<.....>>>><<<<<<.......
....>>>>>>..<<...<<<<<<<...<<<<<<.......>>>>>>....
.<<.[.(((>>....>>>>>>>...<<<<..<<<<....>>>>..>>>>.
<<<....>>>.>>)))]......<<<<<<<.<<..>>>>..>>>>>>>>>
.............>>>>..>>>>>>.......<<<<...<.<<<<...<<
.......>>...>>>>.>....>>>.>.......<<<<<.<<<<<<..<<
<<<<<<<......>>>>>>>..>>.<<<<<.....>>>>>.....>>>>>
..>...>>>.>>....<<<<<<<....>>>.>>>>....>>..>>>>>>>
>>>>....<<<<<<<...<<<<..<<<<<<<...............>>>>
>>>.<<<<<...<<<<...<<<<<<<<<<<))))>>>>>>..>>>>>...
>>>>.>>>>>...<<<....<<<<<...........>>>>>....>>>.>
>>>.......>>>>>>>..<<<<<...>>>>>....<<<<.<<<<.....
..>>>>.....<<<.<<<<.<<<....<<<<<....>>>>>...>>>..>
>>>..>>>...>>>>....>>>>..

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8wi7 Cryo- EM structure of the mycobacterial 70S ribosome in complex with ribosome hibernation promotion factor RafH.
• Resolution: 3.5 Å
• Binding residue (original residue number in PDB): A2 K3 G4 K5 R6 T7 F8 Q9 P10 N11 N12 R13 R14 A16 H19 G20 F21 R22 R28 R37 R38 K40 R42 L45
• Binding residue (residue number reindexed from 1): A1 K2 G3 K4 R5 T6 F7 Q8 P9 N10 N11 R12 R13 A15 H18 G19 F20 R21 R27 R36 R37 K39 R41 L44

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8wi7, PDBe:8wi7, PDBj:8wi7
• PDBsum: 8wi7
• PubMed: 38245551
• UniProt: A0R7K0|RL34_MYCS2 Large ribosomal subunit protein bL34 (Gene Name=rpmH)