Structure of PDB 7nhl Chain 7 Binding Site BS01

Receptor Information

>7nhl Chain 7 (length=42) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

KRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS

Ligand information

>7nhl Chain A (length=2914)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaaugggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuauacggaguuacaaaggacgacauuagacgaaucaucugga
aagaugaaucaaagaagguaauaauccuguagucgaaaauguugucucuc
uugaguggauccugaguacgacggagcacgugaaauuccgucggaaucug
ggaggaccaucuccuaaggcuaaauacucucuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaauaga
accugaaaccgugugcuuacaaguagucagagcccguuaaugggugaugg
cgugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaa
gcaguaaauguggagccguagcgaaagcgagucugaauagggcguuuagu
auuuggucguagacccgaaaccaggugaucuacccuuggucagguugaag
uucagguaacacugaauggaggaccgaaccgacuuacguugaaaagugag
cggaugaacugaggguagcggagaaauuccaaucgaaccuggagauagcu
gguucucuccgaaauagcuuuagggcuagccucaagugaugauuauugga
gguagagcacuguuuggacgaggggccccggguuaccgaauucagacaaa
cuccgaaugccaauuaauuuaacuugggagucagaacaugggugauaagg
uccguguucgaaagggaaacagcccagaccaccagcuaaggucccaaaau
auauguuaaguggaaaaggauguggcguugcccagacaacuaggauguug
gcuuagaagcagccaucauuuaaagagugcguaauagcucacuagucgag
ugacacugcgccgaaaauguaccggggcuaaacauauuaccgaagcugug
gauuguccuuuggacaaugguaggagagcguucuaagggcguugaagcau
gaucguaaggacauguggagcgcuuagaagugagaaugccggugugaguc
gcgaaagacgggugagaaucccguccaccgauugacuaagguuuccagag
gaaggcucguccgcucuggguuagucggguccuaagcugaggccgacagg
cguaggcgauggauaacagguugauauuccuguaccaccuauaaucguuu
uaaucgauggggggacgcaguaggauaggcgaagcgugcgauuggauugc
acgucuaagcaguaaggcugaguauuaggcaaauccgguacucguuaagg
cugagcugugauggggagaagacauugagucuucgagucguugauuucac
acugccgagaaaagccucuagauagaaaauaggugcccguaccgcaaacc
gacacagguagucaagaugagaauucuaaggugagcgagcgaacucucgu
uaaggaacucggcaaaaugaccccguaacuucgggagaaggggugcucuu
uaggguuaacgcccagaagagccgcagugaauaggcccaagcgacuguuu
aucaaaaacacaggucucugcuaaaccguaaggugauguauaggggcuga
cgccugcccggugcuggaagguuaagaggagugguuagcuucugcgaagc
uacgaaucgaagccccaguaaacggcggccguaacuauaacgguccuaag
guagcgaaauuccuugucggguaaguuccgacccgcacgaaaggcguaac
gauuugggcacugucucaacgagagacucggugaaaucauaguaccugug
aagaugcagguuacccgcgacaggacggaaagaccccguggagcuuuacu
guagccugauauugaaauucggcacagcuuguacaggauagguaggagcc
uuugaaacgugagcgcuagcuuacguggaggcgcuggugggauacuaccc
uagcuguguuggcuuucuaacccgcaccacuuaucguggugggagacagu
gucaggcgggcaguuugacuggggcggucgccuccuaaaagguaacggag
gcgcucaaagguucccucagaaugguuggaaaucauucauagaguguaaa
ggcauaagggagcuugacugcgagaccuacaagucgagcagggucgaaag
acggacuuagugauccggugguuccgcauggaagggccaucgcucaacgg
auaaaagcuaccccggggauaacaggcuuaucucccccaagaguucacau
cgacggggagguuuggcaccucgaugucggcucaucgcauccuggggcug
uagucggucccaaggguugggcuguucgcccauuaaagcgguacgcgagc
uggguucagaacgucgugagacaguucggucccuauccgucgugggcgua
ggaaauuugagaggagcuguccuuaguacgagaggaccgggauggacaua
ccucugguguaccaguugucgugccaacggcauagcuggguagcuaugug
uggacgggauaagugcugaaagcaucuaagcaugaagccccccucaagau
gagauuucccaacuucgguuauaagaucccucaaagaugaugagguuaau
agguucgagguggaagcauggugacauguggagcugacgaauacuaaucg
aucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[.(((<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<...<<<<<....
..>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.)))..........
....<<....>>....]].........<<<<....>>>>...........
.......>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...
<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>
>>>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>>
>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.
>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.
....<<<...<<<<<<<<....<.<<<<.>>>>..>....>>>>>>>>..
>>.>.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<<<<......>
>>>>>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....
<<<<<<...........<<..<<<<.<<<<........<<<<<.<<...<
<<<.......>>>>...>>.........<<......>>...>>>>>....
>>>>.>>>>.>>...........))).....>>>>>>.>>>...>>>>>>
><<<<<<<...>>>>>>>....>>>>>><.<<<<<<<.<<......<<<<
<.<<....>>.>>>>>...>>.>>>>>>>..>.....>>>>>><......
<<....<<<<<<.......>>>>>><<<<....<<<<<.<<...<<<<<<
...<<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>
>...>>>...>>...<<<<<........>>>>>))<<<<<<<..<<....
..<<<...<<<<....<<<<..<<<..<<<<<.<<<<<<...(((...>>
>>>><<..<<<<<..<<<<<<<<<<..<<.....>>.>>>>>>.....>>
>>..>>>>>...>>...<<<<<<.....>>>>>>...>>>>>...>>>..
.>>>>.........>>>>..>>>.>>..>>>>>>>>>>>..>>.....<<
......>>>>>>>.<<<.......>>>..>>>>..<<<<....<<<<<<<
<.<<.........<.....<<<<<...............>>>>><<<<<<
<.<<<<....>>>>.>>>>>>>............<<<<<<<<<..<<<..
.......>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<
<<<<.<<<..<<<<<<<..<<<...<<...<<<<<..<<....>>...>>
>>>...>>...>>>>>>>>..>>>>><<<<<<.......>>>>>>.....
...<...........<<<<<<.....>>>>>>.....>....>>>>>..>
>.>>>>>>>..>..>>.>>>>>>>>.>>>>>>.....>.....<<<<<..
......>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<
<<.<<<<<<<<<.<<<<.<<<<<<<<<<<...........<<<<<..<<<
......<<<<<<<<....>>>>>>>>...>>>............>>>>>.
.>>>>>>>>>>>.>>>>....<<.<<<<<<.....>>>>>>>>.....>>
>>>>>>>..>>>>>..<<<<.....>>>><<<<<<...........>>>>
>>..<<...<<<<<<<...<<<<<<.......>>>>>>.....<<.[.((
(>>....>>>>>>>...<<<<..<<<......>>>..>>>>.<<<....>
>>.>>)))]......<<<<<<<.((..>>>>..))>>>>>>>........
.....>>>>..]]>>>>.......<<<<...<<<<<<...<<.......>
>...>>>>>>....>>>.>.......<<<<<.<<<<<<..<<<<<<<<<.
.....>>>>>>..>>>.<<<<<.....>>>>>.....>>>>>..>...>>
>.>>....<<<<<<<....>>>.>>>>....]]..)))>>>>>>>>....
<<<<<<<...<<<<..<<<<<<<...............>>>>>>>.<<<<
<...<<<<...<<<<<<<<<<<.)))>>>>>>..>>>>>...>>>>.>>>
>>...<<<....<<<<<...........>>>>>....>>>.>>>>.....
..>>>>>>>.<<<....>>>....<<<<<<<<<.......>>>>>....<
<<<<<<<.<<<....<<<<<....>>>>>...>>>..>>>>>.>>>...>
>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7nhl Structural basis of ABCF-mediated resistance to pleuromutilin, lincosamide, and streptogramin A antibiotics in Gram-positive pathogens.
• Resolution: 3.1 Å
• Binding residue (original residue number in PDB): K3 R4 T5 Y6 Q7 P8 N9 K10 R11 K12 H13 S14 K15 H17 G18 F19 R20 R22 K26 N27 R34 R35 R36 R37 K38 G39 R40
• Binding residue (residue number reindexed from 1): K1 R2 T3 Y4 Q5 P6 N7 K8 R9 K10 H11 S12 K13 H15 G16 F17 R18 R20 K24 N25 R32 R33 R34 R35 K36 G37 R38

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7nhl, PDBe:7nhl, PDBj:7nhl
• PDBsum: 7nhl
• PubMed: 34117249
• UniProt: Q2FUQ0|RL34_STAA8 Large ribosomal subunit protein bL34 (Gene Name=rpmH)