Structure of PDB 7d63 Chain 5K Binding Site BS01

Receptor Information

>7d63 Chain 5K (length=175) Species: 559292 (Saccharomyces cerevisiae S288C)

TRKFGLVKRTLNTKKDQRLKKNQENLTRNIPQVSSALFFQYNQAIKPPYQ
VLIDTNFINFSIQKKVDIVRGMMDCLLAKCNPLITDCVMAELEKLGPKYR
IALKLARDPRIKRLSCSHKGTYADDCLVHRVLQHKCYIVATNDAGLKQRI
RKIPGIPLMSVGGHAYVIEKLPDVF

Ligand information

>7d63 Chain 3A (length=175)

gucgacguacuucauaggaucauuucuauaggaaucgucacucuuugacu
cuucaaaagagccacugaauccaacuugguugaugagucccauaaccuuu
guacccagugagaaauugccguugcuauggcgcgaugaucacccaugggu
ggguacaaauggcagucugacaagu

..................................................
.......................<<<<<.................<<<<<
<<<<<..<.........<<<<......>>>>........><<<<..>>>>
.>>>>>>>>.>>........>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7d63 Cryo-EM structure of 90S preribosome with inactive Utp24 (state C)
• Resolution: 12.3 Å
• Binding residue (original residue number in PDB): Q23 K133 G134
• Binding residue (residue number reindexed from 1): Q17 K119 G120

Gene Ontology

Molecular Function

• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:7d63, PDBe:7d63, PDBj:7d63
• PDBsum: 7d63
• UniProt: Q05498|FCF1_YEAST rRNA-processing protein FCF1 (Gene Name=FCF1)