Structure of PDB 8h6l Chain 5D Binding Site BS01

Receptor Information

>8h6l Chain 5D (length=1696) Species: 9606 (Homo sapiens)

LPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFK
TLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDG
TINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEE
ISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLE
ALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNS
FRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRK
ETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLP
YGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIK
GTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYY
LSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRM
LRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNF
QVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITV
REEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMA
DMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPL
RQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVH
LFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVD
SEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCE
TQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFN
PIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYI
TPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTP
EKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISS
QIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQG
FNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTT
CAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMER
RLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDY
PIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLD
HCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHR
HLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTI
ELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKL
NNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDV
LSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGV
ESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSI
RSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISI
KRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVD

Ligand information

>8h6l Chain 4A (length=128)

agcuuugcgcaguggcaguaucguagccaaugaggucuauccgaggcgcg
auuauugcuaauuuuuucccccgccgugacgacuugcaauauagucggca
cuggcaauuuuugacagucucgagacug

...................<<<<<.<<<.....<<.....>>..>>>>>>
>>....................<<<<<<.<<<<<........>>>>>.>>
>.>>>.........<<<<<<<>>>>>>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8h6l Structural Insights into Human Exon-defined Spliceosome Prior to Activation
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): M544 R545 T570 T589 E591 I595 K599 K696 H726 S727 R728 K729 A787 T812 A813 T814 Y834 T1028 Y1083 R1090
• Binding residue (residue number reindexed from 1): M115 R116 T141 T160 E162 I166 K170 K267 H297 S298 R299 K300 A358 T383 A384 T385 Y405 T599 Y654 R661

Enzymatic activity

• Enzyme Commision number: 3.6.4.13: RNA helicase.

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0003724 RNA helicase activity
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0000354 cis assembly of pre-catalytic spliceosome
• GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
• GO:0000398 mRNA splicing, via spliceosome
• GO:0001649 osteoblast differentiation
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005681 spliceosomal complex
• GO:0005682 U5 snRNP
• GO:0016020 membrane
• GO:0046540 U4/U6 x U5 tri-snRNP complex
• GO:0071005 U2-type precatalytic spliceosome
• GO:0071006 U2-type catalytic step 1 spliceosome
• GO:0071013 catalytic step 2 spliceosome

External links

• PDB: RCSB:8h6l, PDBe:8h6l, PDBj:8h6l
• PDBsum: 8h6l
• PubMed: 38658629
• UniProt: O75643|U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa helicase (Gene Name=SNRNP200)