Structure of PDB 8h6j Chain 5B Binding Site BS01

Receptor Information
>8h6j Chain 5B (length=2209) Species: 9606 (Homo sapiens) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
MSEEKLQEKARKWQQLQAKRYAEKKEDMPPEHVRKIIRDHDKRVYLGALK
YMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQ
WGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQL
ELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLAN
QLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDW
NEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTE
DPDLPAFYFDPLINPISHREPLPDDDEEFELPEFVEPFLKDTPLYTDNTA
NGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQK
LLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQ
GYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCRE
VLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLM
RQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLE
RWLGNLLARSHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRC
WKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDK
TVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLES
RRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQ
AYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITD
AYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETS
EGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINY
KDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPN
DFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPD
PNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQ
WENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNE
VTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIG
LMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTP
KELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSQLIPNLYRYIQPWE
SEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKD
RHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQ
ALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGL
NQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQI
FRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSC
ADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDI
ERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLI
QQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQI
IWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSV
WAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHL
LDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKT
ISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEW
IKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEIFSSKTEWRVR
AISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAG
YLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLG
WIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLT
AYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQ
SSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVY
SADREDLYA
Ligand information
>8h6j Chain 5A (length=114) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
cucugguuucucuucagaucgcauaaaucuuucgccuuuuacuaaagauu
uccguggagaggaacaacucugagucuuaacccaauuuuuugaggccuug
cuuuggcaaggcua
<<<.<<<<<<<<<<<<........<<<<<<<<...........>>>>>>>
>...>>>>>>>>>>>....>.>>>...................<<<<<<<
<....>>>>>>>>.
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB8h6j Atomic structures of human exon-defined spliceosome prior to activation.
Resolution3.25 Å
Binding residue
(original residue number in PDB)		K101 N221 T224 K267 F279 E280 R284 R409 R417 R419 R420 K428 N457 K460 H461 P463 P464 K465 A466 Q467 K468 K469 R470 R474 Y596 K597 M599 R600 R603 R635 F639 R642 G643 L647 T766
Binding residue
(residue number reindexed from 1)		K58 N178 T181 K224 F236 E237 R241 R360 R368 R370 R371 K379 N408 K411 H412 P414 P415 K416 A417 Q418 K419 K420 R421 R425 Y547 K548 M550 R551 R554 R586 F590 R593 G594 L598 T697
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0070530 K63-linked polyubiquitin modification-dependent protein binding
GO:0097157 pre-mRNA intronic binding
GO:0140492 metal-dependent deubiquitinase activity
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000375 RNA splicing, via transesterification reactions
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0071222 cellular response to lipopolysaccharide
GO:0071356 cellular response to tumor necrosis factor
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0016020 membrane
GO:0016607 nuclear speck
GO:0030532 small nuclear ribonucleoprotein complex
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071007 U2-type catalytic step 2 spliceosome
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8h6j, PDBe:8h6j, PDBj:8h6j
PDBsum8h6j
PubMed38658629
UniProtQ6P2Q9|PRP8_HUMAN Pre-mRNA-processing-splicing factor 8 (Gene Name=PRPF8)

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