Structure of PDB 8p2g Chain 5 Binding Site BS01

Receptor Information

>8p2g Chain 5 (length=53) Species: 93061 (Staphylococcus aureus subsp. aureus NCTC 8325)

AVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCKNCGSYNG
EEV

Ligand information

>8p2g Chain A (length=2844)

uuaaguuauuaagggcgcacgguggaugccuuggcacuagaagccgauga
aggacguuacuaacgacgauaugcuuuggggagcuguaaguaagcuuuga
uccagagauuuccgaauggggaaacccagcaugaguuaugucauguuauc
gauaugugaauacauagcauaucagaaggcacacccggagaacugaaaca
ucuuaguacccggaggaagagaaagaaaauucgauucccuuaguagcggc
gagcgaaacgggaagagcccaaaccaacaagcuugcuuguugggguugua
ggacacucuauacggaguuacaaaggacgacauuagacgaaucaucugga
aagaugaaucaaagaagguaauaauccuguagucgaaaauguugucucuc
uugaguggauccugaguacgacggagcacgugaaauuccgucggaaucug
ggaggaccaucuccuaaggcuaaauacucucuagugaccgauagugaacc
aguaccgugagggaaaggugaaaagcaccccggaaggggagugaaauaga
accugaaaccgugugcuuacaaguagucagagcccguuaaugggugaugg
cgugccuuuuguagaaugaaccggcgaguuacgauuugaugcaagguuaa
gcaguaaauguggagccguagcgaaagcgagucugaauagggcguuuagu
auuuggucguagacccgaaaccaggugaucuacccuuggucagguugaag
uucagguaacacugaauggaggaccgaaccgacuuacguugaaaagugag
cggaugaacugaggguagcggagaaauuccaaucgaaccuggagauagcu
gguucucuccgaaauagcuuuagggcuagccucaagugaugauuauugga
gguagagcacuguuuggacgaggggccguuaccgaauucagacaaacucc
gaaugccaauuaauuuaacuugggagucagaacaugggugauaagguccg
uguucgaaagggaaacagcccagaccaccagcuaaggucccaaaauauau
guuaaguggaaaaggauguggcguugcccagacaacuaggauguuggcuu
agaagcagccaucauuuaaagagugcguaauagcucacuagucgagugac
acugcgccgaaaauguaccggggcuaaacauauuaccgaagcuguggauu
guccuuuggacaaugguaggagagcguucuaagggcguugaagcaugauc
guaaggacauguggagcgcuuagaagugagaaugccggugugaguagcga
aagacgggugagaaucccguccaccgauugacuaagguuuccagaggaag
gcucguccgcucuggguuagucggguccuaagcugaggccgacaggcgua
ggcgauggauaacagguugauauuccuguaccaccuauaaucguuuuaau
cgauggggggacgcaguaggauaggcgaagcgugcgauuggauugcacgu
cuaagcaguaaggcugaguauuaggcaaauccgguacucguuaaggcuga
gcugugauggggagaagacauugugucuucgagucguugauuucacacug
ccgagaaaagccucuagauagaaaauaggugcccguaccgcaaaccgaca
cagguagucaagaugagaauucuaaggugagcgagcgaacucucguuaag
gaacucggcaaaaugaccccguaacuucgggagaaggggugcucuuuagg
guuaacgcccagaagagccgcagugaauaggcccaagcgacuguuuauca
aaaacacaggucucugcuaaaccguaaggugauguauaggggcugacgcc
ugcccggugcuggaagguuaagaggagugguuagcuucugcgaagcuacg
aaucgaagccccaguaaacggcggccguaacuauaacgguccuaagguag
cgaaauuccuugucggguaaguuccgacccgcacgaaaggcguaacgauu
ugggcacugucucaacgagagacucggugaaaucauaguaccugugaaga
ugcagguuacccgcgacaggacggaaagaccccguggagcuuuacuguag
ccugauauugaaauucggcacagcuugcccuagcuguguuggcuuucuaa
cccgcaccacuuaucguggugggagacagugucaggcgggcaguuugacu
ggggcggucgccuccuaaaagguaacggaggcgcucaaagguucccucag
aaugguuggaaaucauucauagaguguaaaggcauaagggagcuugacug
cgagaccuacaagucgagcagggucgaaagacggacuuagugauccggug
guuccgcauggaagggccaucgcucaacggauaaaagcuaccccggggau
aacaggcuuaucucccccaagaguucacaucgacggggagguuuggcacc
ucgaugucggcucaucgcauccuggggcuguagucggucccaaggguugg
gcuguucgcccauuaaagcgguacgcgagcuggguucagaacgucgugag
acaguucggucccuauccgucgugggcguaggaaauuugagaggagcugu
ccuuaguacgagaggaccgggauggacauaccucugguguaccaguuguc
gugccaacggcauagcuggguagcuauguguggacgggauaagugcugaa
agcaucuaagcaugaagccccccucaagaugagauuucccaacuucgguu
auaagaucccucaaagaugaugagguuaauagguucgagguggaagcaug
gugacauguggagcugacgaauacuaaucgaucgaagacuuaau

<<<<<<......<<<<<<<<<<<.....[[.(((<<<<<<<[[<<.....
.<<<<<<.((..((.>>>.....<<<<<<<..<<<))..)).>>>.....
>>>>>>>...>>>....<<<....>>><<<<<<<......>>>>>>>.<<
<<<<<<<.........>>>>>>>.>>.>>....<<<<<......<<....
>>.......>>>>.>.........<<....>>..<<<<<......<<...
..>>.....>>>>>..(((....<<<<<<<<....>>>>>>>>......<
<<<<<<<<.....<<<<.......<<<<<<<<<<.<<<...<<<<<....
..>>>>>((......<<<.....))>>>...>>>...>>>>>>>>>>>>>
>.>>>>>..>>>>.....<<<<<<<<.........>>>>>>>>......<
<<<<..<<<<>>>>>..)))......]].>>>>>>>.)))..........
....<<....>>....]].........<<<<....>>>>...........
.......>>>>>>>>>>>.......<<<<..<<<<......>>>>..>>>
>...<.<........>.>.<<<<<<....<<<<<<<.<<<<<..<<<...
<<<.....>>>...>>>...<<....>>..<<<<.....>>>>.....>>
>>>.>>>>>>>..<<.<...<<<<<<.....<<<<<<<<<..<<<....<
<<<<<......>>>>>>.....>>>...<<<.<<<<<........>>>>>
>>>....>>>>>>>>>...<<<.....>>>......>>>>>>......>.
>>..<<<<<<....<<<<<....>>>>><<<<<<<<<...<<<<<<<<<.
....<<<...<<<<<<<<..................>>>>>>>>..>>.>
.....>>>>.>>>>>..>>>>>>>.>>..<<<<<<<<<<......>>>>>
>>>>>....<<<......>>>...<<<<.<<<...<<<(((.....<<<<
<<...........<<..<<<<.<<<<........<<<<<.<<...<<<<.
......>>>>...>>.........<<......>>...>>>>>....>>>>
.>>>>.>>...........))).....>>>>>>.>>>...>>>>>>><<<
<<<<...>>>>>>>....>>>>>>..<<<<<<<.<<......<<<<<.<<
....>>.>>>>>...>>.>>>>>>>........>>>>>><......<<..
..<<<<<<.......>>>>>><<<<....<<<<<.<<<..<<<<<<...<
<.....>>.>>>>>>....((<<<<.<<...<<<...<<<....>>>...
>>>...>>...<<<<<........>>>>>))<<<<<<<..<<......<<
<...<<<<....<<<<..<<<..<<<<<.<<<<<<...(((...>>>>>>
<<..<<<<<..<<<<<<<<<<..<<.....>>.>>>>>>.....>>>>..
>>>>>...>>...<<<<<<.....>>>>>>...>>>>>...>>>...>>>
>.........>>>>..>>>.>>..>>>>>>>>>>>.>>>.....<<....
..>>>>>>>.<<<<....>.>>>..>>>>..<<<<....<<<<<<<<.<<
...............<<<<<...............>>>>><<<<<<<.<<
<<....>>>>.>>>>>>>............<<<<<<<<<..<<<......
...>>>..<<<<<<<.....<<<....>>>.......>>>>>>>.<<<<<
.<<<..<<<<<<<..<<<<..<<...<<<<<<.<<....>>..>>>>>>.
..>>..>>>>>>>>>..>>>>><<<<<<.......>>>>>>........<
...........<<<<<<.....>>>>>>.....>....>>>>>..>>.>>
>>>>>.....>>.>>>>>>>>.>>>>>>.....>.....<<<<<......
..>>>>>..<<<<<<<<.(((.[[......<<<<.[[<<<<..<<<<<.<
<<<<<<<<.<<<<.<<<<<<<<<<<......>>>>>>>>>>>.>>>>...
.<<.<<<<<<.....>>>>>>>>.....>>>>>>>>>..>>>>>..<<<<
.....>>>><<<<<<...........>>>>>>..<<...<<<<<<<...<
<<<<<.......>>>>>>.....<<.[.(((>>....>>>>>>>...<<<
<..<<<......>>>..>>>>.<<<....>>>.>>)))]......<<<<<
<<.((..>>>>..))>>>>>>>.............>>>>..]]>>>>...
....<<<<...<<<<<<...<<.......>>...>>>>>>....>>>.>.
......<<<<<.<<<<<<..<<<<<<<<<......>>>>>>>..>>.<<<
<<.....>>>>>.....>>>>>..>...>>>.>>....<<<<<<<....>
>>.>>>>....]]..)))>>>>>>>>....<<<<<<<...<<<<..<<<<
<<<...............>>>>>>>.<<<<<...<<<<...<<<<<<<<<
<<.)))>>>>>>..>>>>>...>>>>.>>>>>...<<<....<<<<<...
........>>>>>....>>>.>>>>.......>>>>>>>.<<<....>>>
....<<<<<<<<<.......>>>>>....<<<<<<<<.<<<....<<<<<
....>>>>>...>>>..>>>>>.>>>...>>>>....>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8p2g Cryo-EM structures of Staphylococcus aureus 70S ribosomes in complex with elongation factor G and fusidic acid
• Resolution: 2.02 Å
• Binding residue (original residue number in PDB): A2 V3 P4 K5 R6 R7 T8 S9 K10 T11 R12 K13 K15 R16 R17 T18 H19 F20 T28 P31 S39 H40 R41 Y49 N50
• Binding residue (residue number reindexed from 1): A1 V2 P3 K4 R5 R6 T7 S8 K9 T10 R11 K12 K14 R15 R16 T17 H18 F19 T27 P30 S38 H39 R40 Y48 N49

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8p2g, PDBe:8p2g, PDBj:8p2g
• PDBsum: 8p2g
• PubMed: 38902339
• UniProt: Q2FZF1|RL32_STAA8 Large ribosomal subunit protein bL32 (Gene Name=rpmF)