Structure of PDB 8csq Chain 5 Binding Site BS01

Receptor Information

>8csq Chain 5 (length=319) Species: 9606 (Homo sapiens)

CRLPPLPTIREIIKLLRLQAAKQLSQNFLLDLRLTDKIVRKAGNLTNAYV
YEVGPGPGGITRSILNADVAELLVVEKDTRFIPGLQMLSDAAPGKLRIVH
GDVLTFKVEKAFSESLKRPWEDDPPNVHIIGNLPFSVSTPLIIKWLENIS
CRDGPFVYGRTQMTLTFQKEVAERLAANTGSKQRSRLSVMAQYLCNVRHI
FTIPGQAFVPKPEVDVGVVHFTPLIQPKIEQPFKLVEKVVQNVFQFRRKY
CHRGLRMLFPEAQRLESTGRLLELADIDPTLRPRQLSISHFKSLCDVYRK
MCDEDPQLFAYNFREELKR

Ligand information

>8csq Chain A (length=802)

auagguuugguccuagccuuucuauuagcucuuaguaagauuacacaugc
aagcauccccguuccagugaguucacccucuaaaucaccacgaucaaaag
ggacaagcaucaagcacgcagcaaugcagcucaaaacgcuuagccuagcc
acacccccacgggaaacagcagugauuaaccuuuagcaauaaacgaaagu
uuaacuaagcuauacuaaccccaggguuggucaauuucgugccagccacc
gcggucacacgauuaacccaagucaauagaagccggcguaaagaguguuu
uagaucaaauaaagcuaaaaaaauagacuacgaaaguggcuuuaacauau
cugaacacacaauagcuaagacccaaacugggauuagauaccccacuaug
cuuagcaacaguuaaaucaacaaaacugcucgccagaacacuacgagcca
cagcuuaaaacucaaaggaccuggcggugcuucauaucccucuagaggag
ccuguucuguaaucgauaaaccccgaucaaccucaccaccucuugcucag
ccuauauaccgccaucuuaacccugaugaaggcuacaaaguacguuaggu
caagguguagcccaugagguggcaagaaaugggcuacauuuucuacccca
gaaaauuuagcaguaaacugagaggcuuaguugaacagggcccugaagcg
cgucgucacccuccucaaguauacuucaaaggacauuuaacuaaaacccc
uacgcauuuauauagaggagacaagucguaaaguguacuggaaagugcac
uu

..((((..<<<<<<.)))).<<<<<<.<<..<<<<<<<<..<.<<<.<<<
..<......>......<<.......<<<<.<..<<<.....>>>.>..>>
>>..<<<<....<<...<<<....>>>..>>......>>>>.<<...>>.
>>.<<<....>>>.....>>>>>>>........<<<....<<<<....>>
>>..>>>..>>.>>>>>>.....<<<<<<<<.....(((.....((.>>>
)).......)))..>>>>>..>>.>>>>>>....<<<((.....<<<<..
<<<<.....<<<<<<..............<<....>>.>>>>>>.....>
>>>...>>>>.....<<<<<...<<...<<<<.........>>>>...>>
>>>>>.<.<<<<<......))..>>>>>.>.>>>.<<.......<<<<..
..>>>>.....>>..>>>>>>.....<<<<<<<<...<<<.<<<<<.<<<
<<<<<<<<......<<................>>.<<<<<<<.......<
<.<<<..<<<<<<<.<...<<<<<.....>>>.......>>..>.>.>>>
...>>>>>>>>....>>>>>>>..>>>.>>>>>>>.>...<<<<.....>
>>>..>>>>>.....<<<<<<<...>>>>>>>.....>>>...>>>>>>>
>..<<.....<<<<<<..<<<<<......<<<...<<<.....>>>..>>
>.......>>>>>.>>>>>>......>>..<<<<<<<<<....>>>>>>>
>>

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8csq Principles of mitoribosomal small subunit assembly in eukaryotes.
• Resolution: 2.54 Å
• Binding residue (original residue number in PDB): N36 D40 R42 K86 D111 V112 L113 L142 P143 F144 S145 P149 I152 Q177 E179 V180 R183 S194 R195 L196 Q215 F217 K220 P221 V223 E246 F255 R256 R257 K258 Y259 R291 R293 Q294 R323 L326 K327
• Binding residue (residue number reindexed from 1): N27 D31 R33 K77 D102 V103 L104 L133 P134 F135 S136 P140 I143 Q168 E170 V171 R174 S185 R186 L187 Q206 F208 K211 P212 V214 E237 F246 R247 R248 K249 Y250 R282 R284 Q285 R314 L317 K318

Enzymatic activity

• Enzyme Commision number: 2.1.1.-

Gene Ontology

Molecular Function

• GO:0000179 rRNA (adenine-N6,N6-)-dimethyltransferase activity
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0008168 methyltransferase activity
• GO:0034246 mitochondrial transcription factor activity
• GO:1904047 S-adenosyl-L-methionine binding

Biological Process

• GO:0000154 rRNA modification
• GO:0006364 rRNA processing
• GO:0006391 transcription initiation at mitochondrial promoter
• GO:0031167 rRNA methylation
• GO:0032259 methylation

Cellular Component

• GO:0005739 mitochondrion
• GO:0005759 mitochondrial matrix
• GO:0042645 mitochondrial nucleoid

External links

• PDB: RCSB:8csq, PDBe:8csq, PDBj:8csq
• PDBsum: 8csq
• PubMed: 36482135
• UniProt: Q8WVM0|TFB1M_HUMAN Dimethyladenosine transferase 1, mitochondrial (Gene Name=TFB1M)