Structure of PDB 7suk Chain 5 Binding Site BS01

Receptor Information

>7suk Chain 5 (length=296) Species: 4932 (Saccharomyces cerevisiae)

SVQRDASKGYSILQQTKLFDNIIDLRIKLQKAVIAANKLPLTTESWEEAK
MDDSEETKRLLKENEKLFNNLFNRLINFRIKFQLGDHITQNEEVAKHKLS
KKRSLKELYQETNSLDSELKEYRTAVLNKWSTKVSSASGNAALSSNKFKA
INLPADVQVENQLSDMSRLMKRTKLNRRNITPLYFQKDCANGRLIDITEN
PYVFDDEDFYRVLLNDLIDKKISNAHNSESAAITITSTNARSNNKLKKNI
DTKASKGRKLNYSVQDPIANYEAPITSGYKWSDDQIDEFFAGLLGQ

Ligand information

>7suk Chain 8 (length=1192)

agauaguuaucugguugauccugccaguagucauaugcuugucucagcca
ugcagcucauuaaaucaguuaucguuuauuugauagucaugguauaacug
ugguaauucuagagcuaaaaucucgacccuuuggaagagauguauuuauu
agaucaaugucuucggacucuuugaugaauaacuuuucgaaucgcauggc
cuugugcuggcgaugguucauucaaauuucugcccuaucaacuuucgaug
guaggauaguggccuaccaugguuucaacggguaacggggaauaaggguu
cgauuccggagagggagccugagaaacggcuaccacauccaaggaagggc
aggcuuacccaauccuaauucagggagguagugacaauaaauaacgauac
agggcccauucgggucuuguaauuggaaugaguacaauguaaauaccuua
acgaggaacaauuggagggcaagucuggugccagcagccgcgguaauucc
agcuccaauagcguauauuagcucguaguugaacuauuaauagggacggu
cgggggcaucaguauucaauugucagaggugaaauucuuggauuuauuga
agacuaacuacugcgaaagcauuugccaaggacguuuucauuaaucaaga
acgaaaguuaggggaucgaagaugaucagauaccgucguagucuuaagac
uagggaucgggugguguuuuuuuaaugacccacucggcaccuuacgagaa
aucaaagucuuuggguucuggggggaguauggucgcaaggcugaaacuua
aaggaauugacggaagggcaccaccaggaguggagccugcggcucaacac
ggggaaacucaccagguccagacacaauauuugugggugguggugcaugu
gaugcccuuguucuggcgcgcgcuacacugacggagccagcgagucuaac
cuuggccgagaggucuugguaaaaacuccgucggggaacgaggaauuccu
aguaagcgcaagucaucagcuugcguugauuacgucccugcccuuuguac
acaccgcccgucgcuaguaccgauugaauggcuuagugaggccucaggau
cugcuuagagaagggggcaacuccucucagagcggagauuuggacaaacu
uggucauuuagaggaacuaauuccguaggugaaccugcggaa

................................<<<<<<.....<..<<<.
<<<..<<<.......<<<..<<..<.....<<<..............<<.
.<<.....>>..>>............<<....>>...........<<<<.
..........<....>............>>>>...........<<...<<
.....>>..>>......>>>....>>>..>>>...<....<<<....<<<
<<<<<.......>>>>>>>>>>>......>......<<<...........
....>>>.>>>.<<.<<<..........>>>.>>.<.<<....>>.>.>>
>>>>..<<<....<<<.....>>>..>>>.......>.......<<<<<<
<.<<<<<....>>>>>.>>>.>>>>......<<..<...........>..
>>........<<<<<<<.......<<<....>>>................
..>>>>>>>.>>>>>>......<<..................<..<<<.<
<...<<<.......<<<<.......<<<......>>>..........>>>
>..........<<<....>>>...>>>..>>.>>>...>.........>>
..........<<........<...............>.....>>......
.<<<..<<<<<<<<.<<<........>>>>>>>>>>>..>>>........
........................................<<........
.>>.....<<<<<<<<<<<<..<<.<<<<<<..<<<.<<<<.........
...........<<<<<......<<<<.....>>>>......<<<......
...>>>.....>>>>>>>>>.>>>.....<<<<<<<............<<
<..<<<<....>>>>..>>>.....>>>>>>>............>>>>>>
....<<<<<<<<.......>>>>>>>>......>>...>>>>>>>.....
.......>>>>>..<<<<.<<....<<<<<<<<.<<<....<<......<
<<<<<<.<<<<.<.<......>>.>>>>.>>>>>>>.....>>....>>>
.>>>>>>>>...>>.>>>>..<<<<<<<<....>>>>>>>>.

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7suk Artificial intelligence-assisted cryoEM structure of Bfr2-Lcp5 complex observed in the yeast small subunit processome.
• Resolution: 3.99 Å
• Binding residue (original residue number in PDB): R335 R339 R344 R415 V416 N419 K452 A458 S459 K460 R462
• Binding residue (residue number reindexed from 1): R168 R172 R177 R211 V212 N215 K248 A254 S255 K256 R258

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0015031 protein transport
• GO:0016192 vesicle-mediated transport

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:7suk, PDBe:7suk, PDBj:7suk
• PDBsum: 7suk
• PubMed: 35650250
• UniProt: Q06631|BFR2_YEAST Protein BFR2 (Gene Name=BFR2)